Cytomegalovirus vaccine vector-induced effector memory CD4 + T cells protect cynomolgus macaques from lethal aerosolized heterologous avian influenza challenge.

Autor:	Malouli D; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA., Tiwary M; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA., Gilbride RM; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA., Morrow DW; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA., Hughes CM; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA., Selseth A; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA., Penney T; Tulane National Primate Research Center, Tulane University, New Orleans, LA, USA., Castanha P; Department of Infectious Diseases and Microbiology, Pittsburgh, PA, USA., Wallace M; Department of Infectious Diseases and Microbiology, Pittsburgh, PA, USA., Yeung Y; Department of Infectious Diseases and Microbiology, Pittsburgh, PA, USA., Midgett M; Center for Vaccine Research, Pittsburgh, PA, USA., Williams C; Department of Infectious Diseases and Microbiology, Pittsburgh, PA, USA., Reed J; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA., Yu Y; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA., Gao L; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA., Yun G; Department of Diagnostic Radiology, University of Pittsburgh, Pittsburgh, PA, USA., Treaster L; Department of Diagnostic Radiology, University of Pittsburgh, Pittsburgh, PA, USA., Laughlin A; Center for Vaccine Research, Pittsburgh, PA, USA., Lundy J; Center for Vaccine Research, Pittsburgh, PA, USA., Tisoncik-Go J; Center for Innate Immunity and Immune Disease, University of Washington, Seattle, WA, USA., Whitmore LS; Center for Innate Immunity and Immune Disease, University of Washington, Seattle, WA, USA., Aye PP; Tulane National Primate Research Center, Tulane University, New Orleans, LA, USA., Schiro F; Tulane National Primate Research Center, Tulane University, New Orleans, LA, USA., Dufour JP; Tulane National Primate Research Center, Tulane University, New Orleans, LA, USA., Papen CR; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA., Taher H; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA., Picker LJ; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA., Früh K; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA., Gale M Jr; Center for Innate Immunity and Immune Disease, University of Washington, Seattle, WA, USA.; Washington National Primate Research Center, Seattle, WA, 98195, USA., Maness NJ; Tulane National Primate Research Center, Tulane University, New Orleans, LA, USA., Hansen SG; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA., Barratt-Boyes S; Department of Infectious Diseases and Microbiology, Pittsburgh, PA, USA. smbb@pitt.edu., Reed DS; Center for Vaccine Research, Pittsburgh, PA, USA. dsreed@pitt.edu., Sacha JB; Oregon National Primate Research Center, Vaccine & Gene Therapy Institute, Oregon Health & Science University, Beaverton, OR, USA. sacha@ohsu.edu.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2024 Jul 19; Vol. 15 (1), pp. 6007. Date of Electronic Publication: 2024 Jul 19.
DOI:	10.1038/s41467-024-50345-6
Abstrakt:	An influenza vaccine approach that overcomes the problem of viral sequence diversity and provides long-lived heterosubtypic protection is urgently needed to protect against pandemic influenza viruses. Here, to determine if lung-resident effector memory T cells induced by cytomegalovirus (CMV)-vectored vaccines expressing conserved internal influenza antigens could protect against lethal influenza challenge, we immunize Mauritian cynomolgus macaques (MCM) with cynomolgus CMV (CyCMV) vaccines expressing H1N1 1918 influenza M1, NP, and PB1 antigens (CyCMV/Flu), and challenge with heterologous, aerosolized avian H5N1 influenza. All six unvaccinated MCM died by seven days post infection with acute respiratory distress, while 54.5% (6/11) CyCMV/Flu-vaccinated MCM survived. Survival correlates with the magnitude of lung-resident influenza-specific CD4 + T cells prior to challenge. These data demonstrate that CD4 + T cells targeting conserved internal influenza proteins can protect against highly pathogenic heterologous influenza challenge and support further exploration of effector memory T cell-based vaccines for universal influenza vaccine development. (© 2024. The Author(s).)
Databáze:	MEDLINE
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