Concanavalin A, lectin from Canavalia ensiformis seeds has Leishmania infantum antipromastigote activity mediated by carbohydrate recognition domain.

Autor: Santos ALED; Faculdade de Medicina, Universidade Federal do Cariri, Barbalha, CE, Brazil., Souza ROS; Faculdade de Medicina, Universidade Federal do Cariri, Barbalha, CE, Brazil., Barbosa FEV; Faculdade de Medicina, Universidade Federal do Cariri, Barbalha, CE, Brazil., Santos MHCD; Departamento de Bioquímica, Universidade Federal do Ceará, Fortaleza, CE, Brazil., Grangeiro YA; Departamento de Bioquímica, Universidade Federal do Ceará, Fortaleza, CE, Brazil., Martins AMC; Departamento de Análises Clínicas e toxicológicas, Universidade Federal do Ceará, Fortaleza, CE, Brazil., Santos-Gomes G; Global Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical (IHMT), Universidade Nova de Lisboa, UNL, Lisboa, Portugal., Fonseca IPD; CIISA, Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Av. Universidade Técnica, 1300-477, Lisbon, Portugal; Associate Laboratory for Animal and Veterinary Sciences (AL4AnimalS), Portugal., Silva CGLD; Faculdade de Medicina, Universidade Federal do Cariri, Barbalha, CE, Brazil., Teixeira CS; Faculdade de Medicina, Universidade Federal do Cariri, Barbalha, CE, Brazil; Centro de Ciências Agrárias e da Biodiversidade, Universidade Federal do Cariri, Crato, CE, Brazil. Electronic address: claudener@gmail.com.
Jazyk: angličtina
Zdroj: Chemico-biological interactions [Chem Biol Interact] 2024 Aug 25; Vol. 399, pp. 111156. Date of Electronic Publication: 2024 Jul 18.
DOI: 10.1016/j.cbi.2024.111156
Abstrakt: Leishmaniases, caused by Leishmania parasites, are widespread and pose significant health risks globally. Visceral leishmaniasis (VL) is particularly prevalent in Brazil, with high morbidity and mortality rates. Traditional treatments, such as pentavalent antimonials, have limitations due to toxicity and resistance. Therefore, exploring new compounds like lectins is crucial. Concanavalin A (ConA) has shown promise in inhibiting Leishmania growth. This study aimed to evaluate its leishmanicidal effect on L. infantum promastigotes and understand its mechanism of action. In vitro tests demonstrated inhibition of promastigote growth when treated with ConA, with IC 50 values ranging from 3 to 5 μM over 24-72 h. This study suggests that ConA interacts with L. infantum glycans. Additionally, ConA caused damage to the membrane integrity of parasites and induced ROS production, contributing to parasite death. Scanning electron microscopy confirmed morphological alterations in treated promastigotes. ConA combined with the amphotericin B (AmB) showed synergistic effects, reducing the required dose of AmB, and potentially mitigating its toxicity. ConA demonstrated no cytotoxic effects on macrophages, instead stimulating their proliferation. These findings reinforce that lectin exhibits promising leishmanicidal activity against L. infantum promastigotes, making ConA a potential candidate for leishmaniasis treatment.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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