Engineering of gelatin scaffold by extracellular matrix of Sertoli cells for embryonic stem cell proliferation.

Autor: Keshtmand Z; Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran. Electronic address: Zkeshtmand2001@gmail.com., Eftekhari S; Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran., Khodadadi B; Department of Chemistry, Faculty of Physics and Chemistry, Alzahra University, Tehran, Iran., Farzollahi B; Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran., Khosravimelal S; Department of Medical Biotechnology, Faculty of Allied Medicine, Iran University of Medical Sciences, Tehran, Iran; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran., Shandiz SA; Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran., Tavakkoli Yaraki M; School of Natural Sciences, Faculty of Science and Engineering, Macquarie University, NSW 2109, Australia. Electronic address: mohammad.tavakkoliyaraki@mq.edu.au.
Jazyk: angličtina
Zdroj: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2024 Oct; Vol. 100, pp. 105900. Date of Electronic Publication: 2024 Jul 17.
DOI: 10.1016/j.tiv.2024.105900
Abstrakt: Mimicking the microenvironment of seminiferous tubules plays an indispensable role in directing differentiation of stem cells toward germ cells in vitro. In this work, we fabricated electrospun gelatin (EG) mats (i.e., with diameter <500 nm) conditioned with Sertoli cells' extracellular matrix (ECM) to simulate both 3D structures and composition of normal testis tissue. Sertoli cells were isolated from mice testis and represented through immunocytochemistry (ICC) staining for expression of vimentin, a specific marker of Sertoli cells. The morphological characteristics of ECM-coated scaffold were investigated under scanning electron microscope (SEM). The efficient elimination of cells was confirmed by MTT assay. Furthermore, the cyto/biocompatibility of ECM-conditioned EG scaffold was determined for Sertoli cells and embryonic stem cells (ESCs), alone and as in co-culture. According to the results, the designed scaffold provided a mat for cell proliferation with negligible toxicity (almost 100% cell viability). SEM micrographs displayed cells with elongated shape and complete stretching morphology when compared with those cultured on scaffold without ECM. Moreover, an enhanced differentiation of ESCs toward sperm-generating cells was obtained through co-culturing of Sertoli cells and ESCs, where cell viability was found almost 100%. Our findings introduce the ECM-conditioned EG scaffold as a potentially influential engineered substrate for in vitro guidance of stem cells differentiation by mimicking the native microenvironment.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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