| Autor: |
Lomiwes D; The New Zealand Institute for Plant and Food Research Limited, Palmerston North Campus, 23 Batchelar Road, Fitzherbert, Palmerston North 4410, New Zealand., Günther CS; The New Zealand Institute for Plant and Food Research Limited, Ruakura Campus, Bisley Road, Hamilton 3214, New Zealand., Bloor SJ; Callaghan Innovation, 69 Gracefield Road, P.O. Box 31310, Lower Hutt 5040, New Zealand., Trower TM; The New Zealand Institute for Plant and Food Research Limited, Ruakura Campus, Bisley Road, Hamilton 3214, New Zealand., Ngametua N; The New Zealand Institute for Plant and Food Research Limited, Palmerston North Campus, 23 Batchelar Road, Fitzherbert, Palmerston North 4410, New Zealand., Kanon AP; The New Zealand Institute for Plant and Food Research Limited, Palmerston North Campus, 23 Batchelar Road, Fitzherbert, Palmerston North 4410, New Zealand., Jensen DA; The New Zealand Institute for Plant and Food Research Limited, Ruakura Campus, Bisley Road, Hamilton 3214, New Zealand., Lo K; The New Zealand Institute for Plant and Food Research Limited, Auckland Campus, 120 Mount Albert Road, Sandringham, Auckland 1025, New Zealand., Sawyer G; The New Zealand Institute for Plant and Food Research Limited, Palmerston North Campus, 23 Batchelar Road, Fitzherbert, Palmerston North 4410, New Zealand., Walker EG; The New Zealand Institute for Plant and Food Research Limited, Auckland Campus, 120 Mount Albert Road, Sandringham, Auckland 1025, New Zealand., Hedderley D; The New Zealand Institute for Plant and Food Research Limited, Palmerston North Campus, 23 Batchelar Road, Fitzherbert, Palmerston North 4410, New Zealand., Cooney JM; The New Zealand Institute for Plant and Food Research Limited, Ruakura Campus, Bisley Road, Hamilton 3214, New Zealand. |
| Abstrakt: |
Previous clinical studies indicate that monoamine oxidase-B (MAO-B) inhibition by blackcurrants must be predominantly attributed to bioactives other than anthocyanins. In this natural products discovery study, MAO-A/B inhibitory phytochemicals were isolated from blackcurrants, and a double-blind crossover study investigated the efficacy of freeze-dried whole-fruit blackcurrant powder in inhibiting MAO-B compared with blackcurrant juice in healthy adults. Platelet MAO-B inhibition was comparable between powder (89% ± 6) and juice (91% ± 4), and it was positively correlated with MAO-modulated plasma catecholamines, subjective alertness, and reduced mental fatigue, assessed using the Bond-Lader questionnaire. Sarmentosin, a nitrile glycoside, and its hydroxycinnamoyl esters were identified as novel MAO-A/B inhibitors from blackcurrant in vitro , and sarmentosin was demonstrated to inhibit platelet MAO-B activity in vivo . These findings confirm sarmentosin as the primary bioactive for MAO-A/B inhibition in blackcurrants, as well as its bioavailability and stability during freeze-drying, and suggest that consuming blackcurrant powder and juice may positively affect mood in healthy adults. |
