An ethnopharmacological approach to evaluate antiparasitic and health-promoting abilities of Pueraria tuberosa (Willd.) DC. in livestock.

Autor: Mandal SK; Ethnopharmacology Laboratory, Department of Botany, Visva-Bharati, Santiniketan, India., Mukherjee N; Parasitology Laboratory, Department of Zoology, Visva-Bharati, Santiniketan, India., Ray AS; Ethnopharmacology Laboratory, Department of Botany, Visva-Bharati, Santiniketan, India., Hazra S; Ethnopharmacology Laboratory, Department of Botany, Visva-Bharati, Santiniketan, India., Saha S; Ethnopharmacology Laboratory, Department of Botany, Visva-Bharati, Santiniketan, India., Das S; Division of Microbiology, CSIR-Central Drug Research Institute, Lucknow, Uttar Pradesh, India., Joardar N; Parasitology Laboratory, Department of Zoology, Visva-Bharati, Santiniketan, India., Saha S; Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur, India., Sinha Babu SP; Parasitology Laboratory, Department of Zoology, Visva-Bharati, Santiniketan, India., Rahaman CH; Ethnopharmacology Laboratory, Department of Botany, Visva-Bharati, Santiniketan, India.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Jul 19; Vol. 19 (7), pp. e0305667. Date of Electronic Publication: 2024 Jul 19 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0305667
Abstrakt: In eastern India, the tubers of Pueraria tuberosa (Willd.) DC. are used by the ethnic communities for its wide range of medicinal and nutritional value, especially to rejuvenate livestock health and to treat helminthiasis. The study is aimed to evaluate the ethnoveterinary medicinal importance of P. tuberosa as anthelmintic, to verify its nontoxic nature and identify the most potent phytoconstituents aided by in silico molecular docking technique. Ethnomedicinal data collected from 185 informants were quantitatively analyzed employing eight quantitative indices to highlight the use diversity and most frequently used part of the plant. High scores of certain indices employed, such as Use Value (UV = 0.52), Fidelity Level (FL = 68.42%) and Tissue Importance Value (TIV = 1) clearly illustrate an ethnomedicinal lead regarding medico-nutritional benefits of the tuber part used against intestinal helminthic diseases of veterinary animals. Based on this ethno-guided lead, root tuber has been investigated for its chemical profiling by the estimation of total phenolics, flavonoids, tannins and alkaloids, along with HPLC and GC-MS analyses. Anthelmintic property was evaluated with the tuber extracts by in vitro studies on some helminths of livestock and poultry birds, and it showed promising results against the tested parasites namely Cotylophoron cotylophorum, Raillietina tetragona and Setaria cervi. Toxicity assessments of tuber extract through in vitro and in vivo methods were performed using Vero cells and BALB/c mice. Nontoxic nature of the studied tuber extract was observed even in higher experimental doses. Out of 12 phytocompounds identified by GC-MS analysis, one compound [Morphinan-4,5-epoxy-3,6-di-ol,6- (7-nitrobenzofurazan-4-yl) amino-] exhibited the best binding conformations in cost of the lowest binding energy values with six target proteins that include one anti-inflammatory, one antioxidant, and four anthelmintic proteins. The findings of our study are found very encouraging to evaluate this tuber drug furthermore intensively towards the development of anthelmintic veterinary medicine.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.)
Databáze: MEDLINE
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