The thrombin receptor (PAR1) is associated with microtubules, mitosis and process formation in glioma cells.

Autor:	Golderman V; Department of Neurology, The Chaim Sheba Medical Center, Ramat Gan, 52621, Israel.; Department of Neurology and Neurosurgery, Faculty of Medical and Health Sciences, Tel Aviv University, Tel-Aviv, 6997801, Israel., Gofrit SG; Department of Neurology, The Chaim Sheba Medical Center, Ramat Gan, 52621, Israel., Ivashko-Pachima Y; Elton Laboratory for Molecular Neuroendocrinology, Department of Human Molecular Genetics and Biochemistry, Faculty of Medical and Health Sciences, Adams Super Center for Brain Studies and Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, 6997801, Israel., Gozes I; Elton Laboratory for Molecular Neuroendocrinology, Department of Human Molecular Genetics and Biochemistry, Faculty of Medical and Health Sciences, Adams Super Center for Brain Studies and Sagol School of Neuroscience, Tel Aviv University, Tel Aviv, 6997801, Israel., Chapman J; Department of Neurology, The Chaim Sheba Medical Center, Ramat Gan, 52621, Israel.; Department of Neurology and Neurosurgery, Faculty of Medical and Health Sciences, Tel Aviv University, Tel-Aviv, 6997801, Israel.; Robert and Martha Harden Chair in Mental and Neurological Diseases, Faculty of Medical and Health Sciences, Tel Aviv University, 6997801, Israel., Shavit-Stein E; Department of Neurology, The Chaim Sheba Medical Center, Ramat Gan, 52621, Israel.; Department of Neurology and Neurosurgery, Faculty of Medical and Health Sciences, Tel Aviv University, Tel-Aviv, 6997801, Israel.; The TELEM Rubin Excellence in Biomedical Research Program, The Chaim Sheba Medical Center, Ramat Gan, 52621, Israel.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Jun 19; Vol. 10 (12), pp. e33329. Date of Electronic Publication: 2024 Jun 19 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e33329
Abstrakt:	The cell surface protease-activated receptor 1 (PAR1) is overexpressed in glioblastoma multiforme (GBM). We studied the function and structure of intracellular microtubule (MT) and PAR1 in a tubulin-mediated process. We found that exposure to thrombin increased the percentage of proliferative, S, and M phases cells, affected morphology, and increased process elongation. PAR1 antagonist inversely affects these measures, increases tubulin end-binding protein 3 (EB3) mRNA expression in C6 cells, and reduces EB3 comet length, track length, and duration in neuroblastoma cells. In addition, immunofluorescence staining suggests that PAR1 is in close association with the MT α-tubulin and with coagulation cascade proteins during cell division stages. Our findings support PAR1 involvement in MT dynamics. Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Efrat Shavit-Stein reports financial support was provided by Israel Ministry of Innovation Science & Technology. Efrat Shavit-Stein, Joab Chapman has patent #PAR1 modulating molecules: “Compositions and methods for treating glioblastoma” issued to US-2021228681-A1. (© 2024 The Authors.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu