Control of Coxiella burnetii shedding in a dairy goat herd by annual offspring vaccination.

Autor: Bauer BU; Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany; Institute of Immunology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald - Isle of Riems, Germany. Electronic address: Benjamin.Bauer@fli.de., Herms TL; Food and Veterinary Institute Braunschweig/Hannover, Lower Saxony State Office for Consumer Protection and Food Safety (LAVES), Eintrachtweg 17, 30173 Hannover, Germany. Electronic address: Louise.Herms@LAVES.Niedersachsen.de., Jahnke R; Institute of Immunology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald - Isle of Riems, Germany. Electronic address: Rico.Jahnke@fli.de., Ossowski N; Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany. Electronic address: Nina.Ossowski@tiho-hannover.de., Walter MC; Bundeswehr Institute of Microbiology, Neuherbergstraße 11, 80937 Munich, Germany. Electronic address: MathiasWalter@Bundeswehr.org., Frangoulidis D; Bundeswehr Medical Service Headquarters VI-2, Medical Intelligence & Information (MI2), Dachauer Straße 128, 80637 Munich, Germany. Electronic address: DimitriosFrangoulidis@Bundeswehr.org., Runge M; Food and Veterinary Institute Braunschweig/Hannover, Lower Saxony State Office for Consumer Protection and Food Safety (LAVES), Eintrachtweg 17, 30173 Hannover, Germany. Electronic address: Martin.Runge@LAVES.Niedersachsen.de., Ganter M; Clinic for Swine and Small Ruminants, Forensic Medicine and Ambulatory Service, University of Veterinary Medicine Hannover, Foundation, Bischofsholer Damm 15, 30173 Hannover, Germany. Electronic address: Martin.Ganter@tiho-hannover.de., Knittler MR; Institute of Immunology, Friedrich-Loeffler-Institut, Südufer 10, 17493 Greifswald - Isle of Riems, Germany. Electronic address: Michael.Knittler@fli.de.
Jazyk: angličtina
Zdroj: Vaccine [Vaccine] 2024 Nov 14; Vol. 42 (25), pp. 126125. Date of Electronic Publication: 2024 Jul 18.
DOI: 10.1016/j.vaccine.2024.07.026
Abstrakt: A Coxiella burnetii vaccination program, targeting only doelings, was introduced on a German goat farm to curb bacterial shedding. In 2018, adults were vaccinated with a C. burnetii Phase I vaccine at three-weeks apart following pathogen diagnosis, with a booster administered six months later due to sustained high shedding. From 2018 to 2021, doelings received two vaccine doses without any further boosters. To assess the program's efficacy, vaginal swabs from up to 40 animals per age group were collected during kidding seasons from 2019 to 2022. Bulk tank milk (BTM) samples were gathered monthly from January 2018 to October 2022 to monitor herd-level shedding. Real-time PCR analysis determined genome equivalents in all three sample types. Serum samples were taken before the initial immunization and during the post-kidding season from up to 40 goats per age group annually from 2018 to 2022. Phase-specific ELISAs determined IgG Phase I and Phase II antibodies. Additionally, two serum samples per age group from 2022 were analyzed using a neutralization assay. A few goats continued shedding small quantities during subsequent kidding seasons. Although positive BTM samples decreased, they displayed an undulating trend. Most age groups exhibited robust IgG Phase I responses and lower IgG Phase II levels post immunization. Mean IgG levels remained elevated until the study ended compared to pre-vaccination levels in most age groups. Additionally, neutralizing antibodies were present regardless of IgG response. Overall, double vaccination induced lasting antibody levels, but did not entirely prevent C. burnetii shedding. The resilience of the observed humoral immune activity requires further investigation.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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