The porcine corpus luteum as a model for studying the effects of nanoplastics.

Autor: Basini G; Dipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, Via del Taglio 10, Parma 43126, Italy. Electronic address: basini@unipr.it., Bertini S; Dipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, Via del Taglio 10, Parma 43126, Italy., Bussolati S; Dipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, Via del Taglio 10, Parma 43126, Italy., Zappavigna F; Dipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, Via del Taglio 10, Parma 43126, Italy., Berni M; Risk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Strada dei Mercati 13a, Parma 43126, Italy., Scaltriti E; Risk Analysis and Genomic Epidemiology Unit, Istituto Zooprofilattico Sperimentale della Lombardia e dell'Emilia Romagna, Strada dei Mercati 13a, Parma 43126, Italy., Ramoni R; Dipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, Via del Taglio 10, Parma 43126, Italy., Grolli S; Dipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, Via del Taglio 10, Parma 43126, Italy., Quintavalla F; Dipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, Via del Taglio 10, Parma 43126, Italy., Grasselli F; Dipartimento di Scienze Medico-Veterinarie, Università degli Studi di Parma, Via del Taglio 10, Parma 43126, Italy.
Jazyk: angličtina
Zdroj: Environmental toxicology and pharmacology [Environ Toxicol Pharmacol] 2024 Sep; Vol. 110, pp. 104503. Date of Electronic Publication: 2024 Jul 24.
DOI: 10.1016/j.etap.2024.104503
Abstrakt: Nanoplastics (NPs) affect fertility. We evaluated the effects of NPs treatment on luteal and endothelial cells. We examined crucial markers of growth and redox status. NPs treatment did not induce changes in ATP levels in luteal cells, while it increased (p< 0.05) their proliferation. In endothelial cells, no change in proliferation was detected, while an increase (p<0.05) in ATP levels was observed. The increase of reactive oxygen species, superoxide anion (p<0.05) and nitric oxide (p<0.001) was detected in both cell types, which also showed changes in superoxide dismutase enzyme activity as well as an increase of non-enzymatic antioxidant power (p<0.05). A decrease (p<0.05) in progesterone production as well as an increase of vascular endothelial growth factor A levels were detected (p<0.05). In addition, a dose-dependent accumulation of NPs in endothelial cells was shown, that likely occurred through adhesion and internalization. Results underline potential risk of NPs for corpus luteum functionality.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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