Pro-inflammatory and genotoxic responses by metal oxide nanomaterials in alveolar epithelial cells and macrophages in submerged condition and air-liquid interface: An in vitro-in vivo correlation study.

Autor: Di Ianni E; Department of Neurology, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA; National Research Centre for the Working Environment, DK-2100 Copenhagen, Copenhagen, Denmark., Erdem JS; National Institute of Occupational Health, Oslo, Norway., Narui S; National Institute of Occupational Health, Oslo, Norway., Wallin H; National Institute of Occupational Health, Oslo, Norway., Lynch I; School of Geography, Earth and Environmental Sciences, University of Birmingham, Edgbaston, Birmingham B15 2TT, United Kingdom., Vogel U; National Research Centre for the Working Environment, DK-2100 Copenhagen, Copenhagen, Denmark; DTU Food, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark., Jacobsen NR; National Research Centre for the Working Environment, DK-2100 Copenhagen, Copenhagen, Denmark., Møller P; Section of Environmental Health, Department of Public Health, University of Copenhagen, Copenhagen, Denmark. Electronic address: pemo@sund.ku.dk.
Jazyk: angličtina
Zdroj: Toxicology in vitro : an international journal published in association with BIBRA [Toxicol In Vitro] 2024 Oct; Vol. 100, pp. 105897. Date of Electronic Publication: 2024 Jul 25.
DOI: 10.1016/j.tiv.2024.105897
Abstrakt: Studies on in vitro-in vivo correlations of inflammatory and genotoxic responses are needed to advance new approach methodologies. Here, we assessed pro-inflammatory and genotoxic responses by 13 nanosized metal oxides (nMeOx) and quartz (DQ12) in alveolar epithelial cells (A549) and macrophages (THP-1a) exposed in submerged conditions, and in A549:THP-1a co-cultures in air-liquid interface (ALI) system. Soluble nMeOx produced the highest IL-8 expression in A549 and THP-1a cells in submerged conditions (≥2-fold, p < 0.05), whereas only CuO caused a strong response in co-cultures exposed in the ALI system (13-fold, p < 0.05). IL-8 expression in A549 cells with concentrations as nMeOx specific surface area (SSA) correlated with neutrophil influx in mice (r = 0.89-0.98, p < 0.05). Similarly, IL-8 expression in THP-1a cell with concentrations as mass and SSA (when excluding soluble nMeOx) correlated with neutrophil influx in mice (r = 0.81-0.84, p < 0.05). DNA strand breaks (SB) was measured by the comet assay. We used a scoring system that categorizes effects in standard deviation units for comparison of genotoxicity in different models. Concordant genotoxicity was observed between SB levels in vitro (A549 and co-culture) and in vivo (broncho-alveolar lavage fluid cells and lung tissue). In conclusion, this study shows in vitro-in vivo correlations of nMeOx-induced inflammatory and genotoxic responses.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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