A case of asymptomatic infection of Ascaris suum identified by PCR-restriction fragment length polymorphism and DNA sequence analysis.

Autor: Nishioka M; Division of Laboratory, Yamaguchi University Hospital, Ube 755-8505, Japan. Electronic address: mnishi@yamaguchi-u.ac.jp., Hamabe K; Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan., Kunimune Y; Division of Laboratory, Yamaguchi University Hospital, Ube 755-8505, Japan., Kodama M; Division of Laboratory, Yamaguchi University Hospital, Ube 755-8505, Japan., Nakahara Y; Division of Laboratory, Yamaguchi University Hospital, Ube 755-8505, Japan., Okayama N; Division of Medical Genetics, Yamaguchi University Hospital, Ube 755-8505, Japan., Tominaga M; Division of Laboratory, Yamaguchi University Hospital, Ube 755-8505, Japan., Furutani H; Division of Laboratory, Yamaguchi University Hospital, Ube 755-8505, Japan., Ito F; Division of Laboratory, Yamaguchi University Hospital, Ube 755-8505, Japan., Fujii T; Division of Laboratory, Yamaguchi University Hospital, Ube 755-8505, Japan., Suehiro Y; Division of Laboratory, Yamaguchi University Hospital, Ube 755-8505, Japan; Division of Medical Genetics, Yamaguchi University Hospital, Ube 755-8505, Japan; Department of Oncology and Laboratory Medicine, Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan., Takami T; Department of Gastroenterology and Hepatology, Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan., Yamasaki T; Division of Laboratory, Yamaguchi University Hospital, Ube 755-8505, Japan; Department of Oncology and Laboratory Medicine, Yamaguchi University Graduate School of Medicine, Ube 755-8505, Japan.
Jazyk: angličtina
Zdroj: Diagnostic microbiology and infectious disease [Diagn Microbiol Infect Dis] 2024 Sep; Vol. 110 (1), pp. 116444. Date of Electronic Publication: 2024 Jul 14.
DOI: 10.1016/j.diagmicrobio.2024.116444
Abstrakt: Human ascariasis is a soil-transmitted helminthiasis and remains a neglected tropical disease. Ascaris suum has the potential to cause cross-infections between humans and pigs. In this study, we present a rare case of a patient with asymptomatic infection by Ascaris suum. A 66-year-old male underwent colonoscopy, and a white linear worm body was found in the hepatic curvature. The worm was collected by aspiration and submitted to the laboratory for parasite identification. The patient had no symptoms related to parasitic infection. The worm was highly suspected to be of the genus Ascaris. Because of the difficulty of morphological classification, genetic analysis was performed. From PCR-restriction fragment length polymorphism results and sequence analysis of the internal transcribed spacer-1 region, it was determined to be A. suum. The experience with rapid differentiation of A. suum by performing genetic analysis will be useful for future examinations of parasitic infections.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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