Metabolic syndrome induces benefits in mice experiencing severe sepsis, comparable to the effects of low-dose aspirin pretreatment in septic mice lacking metabolic syndrome.

Autor: Nakama RP; Laboratory of Experimental Immunopathology, Department of Immunology, Parasitology and General Pathology, Center for Biological Sciences, State University of Londrina, PR, Brazil., Dos Santos LF; Laboratory of Microorganism Molecular Biology, Department of Microbiology, Center for Biological Sciences, State University of Londrina, PR, Brazil., Berto-Pereira L; Laboratory of Cardiovascular Physiology and Physiopathology, Department of Physiological Sciences, Center for Biological Sciences, State University of Londrina, PR, Brazil., de Rossi LS; Laboratory of Experimental Immunopathology, Department of Immunology, Parasitology and General Pathology, Center for Biological Sciences, State University of Londrina, PR, Brazil., Malvezi AD; Laboratory of Experimental Immunopathology, Department of Immunology, Parasitology and General Pathology, Center for Biological Sciences, State University of Londrina, PR, Brazil., Lovo-Martins MI; Laboratory of Experimental Immunopathology, Department of Immunology, Parasitology and General Pathology, Center for Biological Sciences, State University of Londrina, PR, Brazil., Canizares Cardoso AP; Laboratory of Experimental Immunopathology, Department of Immunology, Parasitology and General Pathology, Center for Biological Sciences, State University of Londrina, PR, Brazil., de Freitas AMD; Laboratory of Pharmacology of Inflammation, Department of Physiological Sciences, Center for Biological Sciences, State University of Londrina, PR, Brazil., Martins-Pinge MC; Laboratory of Experimental Immunopathology, Department of Immunology, Parasitology and General Pathology, Center for Biological Sciences, State University of Londrina, PR, Brazil; Laboratory of Cardiovascular Physiology and Physiopathology, Department of Physiological Sciences, Center for Biological Sciences, State University of Londrina, PR, Brazil., Pinge-Filho P; Laboratory of Experimental Immunopathology, Department of Immunology, Parasitology and General Pathology, Center for Biological Sciences, State University of Londrina, PR, Brazil; Laboratory of Microorganism Molecular Biology, Department of Microbiology, Center for Biological Sciences, State University of Londrina, PR, Brazil. Electronic address: pingefilho@uel.br.
Jazyk: angličtina
Zdroj: International immunopharmacology [Int Immunopharmacol] 2024 Sep 30; Vol. 139, pp. 112694. Date of Electronic Publication: 2024 Jul 17.
DOI: 10.1016/j.intimp.2024.112694
Abstrakt: Background: Sepsis is a complex condition characterized by systemic host inflammation caused by an infection. Experimental and observational studies indicate that obesity, one of the components of metabolic syndrome (MetS), or aspirin (ASA) treatment could be associated with sepsis survival. However, the effects of ASA on septic mice with MetS-induced conditions have not been explored.
Methods: Swiss mice were administered monosodium glutamate (MSG) (4 mg/kg) during their first 5 days of life for MetS induction, while the control mice received an equimolar saline solution. MetS was validated in male mice on their 60th day of life. ASA treatment was administered for 15 days prior to sepsis (40 mg/kg). On the 75th day, sepsis was induced in MetS and control mice through cecal ligation and puncture (CLP). The effects of ASA on septic mice with MSG-induced MetS were assessed by determining survival rates, quantification of nitric oxide (NO), and cytokine levels in the plasma, while correlating these data with hematological, blood glucose and cardiovascular parameters.
Results: MetS was validated by Lee-Index (3 body weight/naso-anal length×1000), hypertension, and hyperglycemia in animals receiving MSG as neonates. In control animals, severe sepsis promoted hypoglycemia, which was associated with mortality, as well as increased plasma NO levels, hypotension, hematological alterations, and elevation of proinflammatory cytokines. In contrast, MetS and pre-treatment with ASA were able to prevent sepsis-related alterations.
Conclusions: MetS and ASA pre-treatment provided protection against severe sepsis. However, ASA was ineffective in mice with MetS undergoing severe sepsis.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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