Development of PainFace software to simplify, standardize, and scale up mouse grimace analyses.

Autor:	McCoy ES; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.; Departments of Cell Biology & Physiology and., Park SK; Psychiatry, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States., Patel RP; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States., Ryan DF; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.; Departments of Cell Biology & Physiology and., Mullen ZJ; Kitware, Inc, Carrboro, NC, United States., Nesbitt JJ; Kitware, Inc, Carrboro, NC, United States., Lopez JE; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States., Taylor-Blake B; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.; Departments of Cell Biology & Physiology and., Vanden KA; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States., Krantz JL; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.; Departments of Cell Biology & Physiology and., Hu W; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.; Departments of Cell Biology & Physiology and., Garris RL; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.; Departments of Cell Biology & Physiology and., Snyder MG; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States., Lima LV; Departments of Psychology and Anesthesia, Alan Edwards Centre for Research on Pain, McGill University, Montreal, QC, Canada., Sotocinal SG; Departments of Psychology and Anesthesia, Alan Edwards Centre for Research on Pain, McGill University, Montreal, QC, Canada., Austin JS; Departments of Psychology and Anesthesia, Alan Edwards Centre for Research on Pain, McGill University, Montreal, QC, Canada., Kashlan AD; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States., Shah S; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States., Trocinski AK; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States., Pudipeddi SS; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States., Major RM; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States., Bazick HO; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States., Klein MR; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States., Mogil JS; Departments of Psychology and Anesthesia, Alan Edwards Centre for Research on Pain, McGill University, Montreal, QC, Canada., Wu G; Psychiatry, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.; Department of Computer Science, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States., Zylka MJ; UNC Neuroscience Center, The University of North Carolina at Chapel Hill, Chapel Hill, NC, United States.; Departments of Cell Biology & Physiology and.
Jazyk:	angličtina
Zdroj:	Pain [Pain] 2024 Aug 01; Vol. 165 (8), pp. 1793-1805. Date of Electronic Publication: 2024 Feb 13.
DOI:	10.1097/j.pain.0000000000003187
Abstrakt:	Abstract: Facial grimacing is used to quantify spontaneous pain in mice and other mammals, but scoring relies on humans with different levels of proficiency. Here, we developed a cloud-based software platform called PainFace ( http://painface.net ) that uses machine learning to detect 4 facial action units of the mouse grimace scale (orbitals, nose, ears, whiskers) and score facial grimaces of black-coated C57BL/6 male and female mice on a 0 to 8 scale. Platform accuracy was validated in 2 different laboratories, with 3 conditions that evoke grimacing-laparotomy surgery, bilateral hindpaw injection of carrageenan, and intraplantar injection of formalin. PainFace can generate up to 1 grimace score per second from a standard 30 frames/s video, making it possible to quantify facial grimacing over time, and operates at a speed that scales with computing power. By analyzing the frequency distribution of grimace scores, we found that mice spent 7x more time in a "high grimace" state following laparotomy surgery relative to sham surgery controls. Our study shows that PainFace reproducibly quantifies facial grimaces indicative of nonevoked spontaneous pain and enables laboratories to standardize and scale-up facial grimace analyses. (Copyright © 2024 International Association for the Study of Pain.)
Databáze:	MEDLINE
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