Virus-like particles displaying the mature C-terminal domain of filamentous hemagglutinin are immunogenic and protective against Bordetella pertussis respiratory infection in mice.

Autor:	Pyles GM; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Huckaby AB; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Gutierrez MdlP; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Witt WT; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Mateu-Borrás M; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Dublin SR; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Rocuskie-Marker C; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Sesti BN; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Peasak K; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Bitzer GJ; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Rader N; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Weaver KL; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Boehm DT; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Fitzgerald N; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Chapman J; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Ulicny S; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Damron FH; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA., Barbier M; Department of Microbiology, Immunology and Cell Biology, West Virginia University , Morgantown, West Virginia, USA.; Vaccine Development Center, West Virginia University Health Sciences Center , Morgantown, West Virginia, USA.
Jazyk:	angličtina
Zdroj:	Infection and immunity [Infect Immun] 2024 Aug 13; Vol. 92 (8), pp. e0027024. Date of Electronic Publication: 2024 Jul 18.
DOI:	10.1128/iai.00270-24
Abstrakt:	Bordetella pertussis, the bacterium responsible for whooping cough, remains a significant public health challenge despite the existing licensed pertussis vaccines. Current acellular pertussis vaccines, though having favorable reactogenicity and efficacy profiles, involve complex and costly production processes. In addition, acellular vaccines have functional challenges such as short-lasting duration of immunity and limited antigen coverage. Filamentous hemagglutinin (FHA) is an adhesin of B. pertussis that is included in all multivalent pertussis vaccine formulations. Antibodies to FHA have been shown to prevent bacterial attachment to respiratory epithelial cells, and T cell responses to FHA facilitate cell-mediated immunity. In this study, FHA's mature C-terminal domain (MCD) was evaluated as a novel vaccine antigen. MCD was conjugated to virus-like particles via SpyTag-SpyCatcher technology. Prime-boost vaccine studies were performed in mice to characterize immunogenicity and protection against the intranasal B. pertussis challenge. MCD-SpyVLP was more immunogenic than SpyTag-MCD antigen alone, and in Tohama I strain challenge studies, improved protection against challenge was observed in the lungs at day 3 and in the trachea and nasal wash at day 7 post-challenge. Furthermore, a B. pertussis strain encoding genetically inactivated pertussis toxin was used to evaluate MCD-SpyVLP vaccine immunity. Mice vaccinated with MCD-SpyVLP had significantly lower respiratory bacterial burden at both days 3 and 7 post-challenge compared to mock-vaccinated animals. Overall, these data support the use of SpyTag-SpyCatcher VLPs as a platform for use in vaccine development against B. pertussis and other pathogens. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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