Development of an aptasensor for highly sensitive detection of cardiac troponin I using cobalt-nickel metal-organic framework (CoNi-MOF).

Autor:	Devaraj R; Department of Electrical & Electronics Engineering, PSG College of Technology, Coimbatore, India., Loganathan AK; Department of Electrical & Electronics Engineering, PSG College of Technology, Coimbatore, India., Krishnamoorthy L; Department of Electrical & Electronics Engineering, PSG College of Technology, Coimbatore, India.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 Jun 19; Vol. 10 (12), pp. e33238. Date of Electronic Publication: 2024 Jun 19 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e33238
Abstrakt:	Objective and Rationale: This study aimed to develop a highly sensitive and selective single-stranded DNA (ssDNA) aptamer targeting cardiac troponin I (cTnI), a crucial biomarker for acute myocardial infarction (AMI). The objective was to fabricate a novel aptamer electrochemical sensor using a composite material of cobalt-nickel metal-organic framework (CoNi-MOF) on screen-printed carbon electrodes (SPCE), leveraging the composite's large surface area and excellent electrical conductivity alongside the aptamer's high affinity for cTnI. Methods: The aptamer electrochemical sensor was fabricated using the CoNi-MOF composite on SPCE and characterized its properties. They conducted electrochemical measurements to assess the sensor's performance in detecting cTnI. The sensor's stability, reproducibility, and electro-catalytic activity were evaluated. Results: The sensor demonstrated linear detection of cTnI over a concentration range of 5-75 pg/mL, with a low limit of detection (LOD) of 13.2 pM. Remarkable stability and reproducibility were observed in cTnI detection. The sensor exhibited exceptional electro-catalytic activity, enabling accurate quantification of cTnI levels in various solutions. Conclusions: This research presents a significant advancement towards the development of reliable, cost-effective, and easily deployable cTnI sensors for clinical applications. The sensor's versatility in detecting cTnI across different concentration ranges highlights its potential utility in diverse clinical settings, particularly for early detection and monitoring of cardiac conditions. Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: AshokKumar Loganathan reports financial support was provided by 10.13039/501100001411Indian Council of Medical Research. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. (© 2024 The Author(s).)
Databáze:	MEDLINE
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