GC-MS analysis and pharmacological potentiality of Lasia spinosa (L.) Thwaites leaves and fruit extracts: an in vitro and in silico studies.

Autor: Haq MA; Professor Joardar DNA and Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, -6205 Bangladesh., Hosen ME; Professor Joardar DNA and Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, -6205 Bangladesh.; Department of Microbiology, Shaheed Shamsuzzoha Institute of Biosciences, Affiliated with University of Rajshahi, Rajshahi, -6212 Bangladesh., Rani R; Professor Joardar DNA and Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, -6205 Bangladesh., Hossain A; Professor Joardar DNA and Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, -6205 Bangladesh., Rahman M; Department of Microbiology, Rajshahi Institute of Biosciences, Affiliated with University of Rajshahi, Rajshahi, Bangladesh., Ali MS; Department of Food and Nutritional Science, Rajshahi Institute of Biosciences, Affiliated with University of Rajshahi, Rajshahi, Bangladesh., Zaman R; Professor Joardar DNA and Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, -6205 Bangladesh., Khalekuzzaman M; Professor Joardar DNA and Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, -6205 Bangladesh., Islam MA; Professor Joardar DNA and Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, -6205 Bangladesh., Dutta AK; Department of Microbiology, University of Rajshahi, Rajshahi, -6205 Bangladesh., Acharjee UK; Professor Joardar DNA and Chromosome Research Laboratory, Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi, -6205 Bangladesh.
Jazyk: angličtina
Zdroj: In silico pharmacology [In Silico Pharmacol] 2024 Jul 15; Vol. 12 (2), pp. 61. Date of Electronic Publication: 2024 Jul 15 (Print Publication: 2024).
DOI: 10.1007/s40203-024-00235-y
Abstrakt: Lasia spinosa ( L. spinosa ) is widely used in Asian countries for treating various diseases and as a vegetable, yet its bioactive properties remain under-researched. It is traditionally utilized in Ayurveda and the AYUSH system of medicine for its medicinal properties, and commonly used to treat digestive disorders, respiratory issues, and inflammatory conditions. This study aims to identify the phytochemicals in L. spinosa leaves and fruit extracts and evaluate their biological activities. Phytochemicals in methanol extracts of L. spinosa fruits and leaves were identified by GC-MS analysis. Antioxidant and cytotoxic activities were assessed using the DPPH free radical and nitric oxide (NO) scavenging assay and brine shrimp lethality test. Antibacterial activity was evaluated against Shigella boydii , Shigella flexneri , Streptococcus iniae , and Streptococcus dysgalactiae , while antifungal properties were tested against Cercospora beticola and Rhizoctonia solani . Molecular docking was conducted to predict the effectiveness of L. spinosa phytochemicals against NADPH oxidase and the Shigella effector OspG. Nine compounds were detected from both extracts. The methanol leaves extract exhibited superior antioxidant activity compared to the fruit extract, with IC 50 values of 111.81 ± 8.99 µg/ml and 174.81 ± 4.86 µg/ml, respectively, as determined by the DPPH scavenging assay. The nitric oxide (NO) scavenging assay also revealed higher potency in the leaves extract (IC 50  = 138.59 ± 1.50 µg/ml) compared to the fruit extract (IC 50  = 196.47 ± 1.72 µg/ml). Both extracts showed significant antimicrobial activity against all tested microorganisms. In silico studies indicated notable inhibitory activity of all phytochemicals against the target proteins, with Linoelaidic acid and 9-Octadecenamide, (Z)- exhibiting the highest activity against NADPH oxidase (PDB: 2cdu) and Shigella flexneri OspG effector kinase (PDB: 4bvu), respectively. These findings suggest that L. spinosa has potent antioxidant and antimicrobial activities. Compounds from this plant could serve as lead compounds for developing antioxidant and antibacterial agents. However, molecular studies should be addressed.
Competing Interests: Competing interestsThe authors declare no competing interests.
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Databáze: MEDLINE
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