Gut microbiome in endometriosis: a cohort study on 1000 individuals.

Autor: Pérez-Prieto I; Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Granada, Spain. inmapprieto@ugr.es.; Instituto de Investigación Biosanitaria Ibs.GRANADA, Granada, Spain. inmapprieto@ugr.es., Vargas E; Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Granada, Spain.; Instituto de Investigación Biosanitaria Ibs.GRANADA, Granada, Spain.; Systems Biology Unit, Department of Experimental Biology, Faculty of Experimental Sciences, University of Jaen, Jaen, Spain., Salas-Espejo E; Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Granada, Spain., Lüll K; Institute of Genomics, Estonian Genome Centre, University of Tartu, Tartu, Estonia., Canha-Gouveia A; Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Granada, Spain.; Instituto de Investigación Biosanitaria Ibs.GRANADA, Granada, Spain.; Department of Physiology, Faculty of Veterinary, University of Murcia, IMIB-Arrixaca, Campus Mare Nostrum, Murcia, Spain., Pérez LA; Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Granada, Spain.; Department of Computer Engineering, Automation and Robotics, University of Granada, Granada, Spain., Fontes J; Instituto de Investigación Biosanitaria Ibs.GRANADA, Granada, Spain.; U. Reproducción, UGC Laboratorio Clínico y UGC Obstetricia y Ginecología. HU Virgen de Las Nieves, Granada, Spain., Salumets A; Competence Centre On Health Technologies, Tartu, Estonia.; Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden.; Department of Obstetrics and Gynaecology, Institute of Clinical Medicine, University of Tartu, Tartu, Estonia.; Institute of Molecular and Cell Biology, University of Tartu, Tartu, Estonia., Andreson R; Institute of Genomics, Estonian Genome Centre, University of Tartu, Tartu, Estonia.; Institute of Genomics, Estonian Genome Center, University of Tartu, Tartu, Estonia., Aasmets O; Institute of Genomics, Estonian Genome Centre, University of Tartu, Tartu, Estonia., Whiteson K; School of Biological Sciences, University of California, Irvine, CA, USA., Org E; Institute of Genomics, Estonian Genome Centre, University of Tartu, Tartu, Estonia., Altmäe S; Department of Biochemistry and Molecular Biology I, Faculty of Sciences, University of Granada, Granada, Spain.; Instituto de Investigación Biosanitaria Ibs.GRANADA, Granada, Spain.; Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden.; Department of Obstetrics and Gynaecology, Institute of Clinical Medicine, University of Tartu, Tartu, Estonia.
Jazyk: angličtina
Zdroj: BMC medicine [BMC Med] 2024 Jul 18; Vol. 22 (1), pp. 294. Date of Electronic Publication: 2024 Jul 18.
DOI: 10.1186/s12916-024-03503-y
Abstrakt: Background: Endometriosis, defined as the presence of endometrial-like tissue outside of the uterus, is one of the most prevalent gynecological disorders. Although different theories have been proposed, its pathogenesis is not clear. Novel studies indicate that the gut microbiome may be involved in the etiology of endometriosis; nevertheless, the connection between microbes, their dysbiosis, and the development of endometriosis is understudied. This case-control study analyzed the gut microbiome in women with and without endometriosis to identify microbial targets involved in the disease.
Methods: A subsample of 1000 women from the Estonian Microbiome cohort, including 136 women with endometriosis and 864 control women, was analyzed. Microbial composition was determined by shotgun metagenomics and microbial functional pathways were annotated using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database. Partitioning Around Medoids (PAM) algorithm was performed to cluster the microbial profile of the Estonian population. The alpha- and beta-diversity and differential abundance analyses were performed to assess the gut microbiome (species and KEGG orthologies (KO)) in both groups. Metagenomic reads were mapped to estrobolome-related enzymes' sequences to study potential microbiome-estrogen metabolism axis alterations in endometriosis.
Results: Diversity analyses did not detect significant differences between women with and without endometriosis (alpha-diversity: all p-values > 0.05; beta-diversity: PERMANOVA, both R 2  < 0.0007, p-values > 0.05). No differential species or pathways were detected after multiple testing adjustment (all FDR p-values > 0.05). Sensitivity analysis excluding women at menopause (> 50 years) confirmed our results. Estrobolome-associated enzymes' sequence reads were not significantly different between groups (all FDR p-values > 0.05).
Conclusions: Our findings do not provide enough evidence to support the existence of a gut microbiome-dependent mechanism directly implicated in the pathogenesis of endometriosis. To the best of our knowledge, this is the largest metagenome study on endometriosis conducted to date.
(© 2024. The Author(s).)
Databáze: MEDLINE
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