Electrokinetic convection-enhanced delivery for infusion into the brain from a hydrogel reservoir.

Autor: Cruz-Garza JG; Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA. jgcruzgarza@houstonmethodist.org.; Center for Neural Systems Restoration, Houston Methodist Research Institute, Houston, TX, USA. jgcruzgarza@houstonmethodist.org., Bhenderu LS; Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA. lokeshwar.bhenderu@houstonmethodist.org.; Center for Neural Systems Restoration, Houston Methodist Research Institute, Houston, TX, USA. lokeshwar.bhenderu@houstonmethodist.org.; Texas A&M University College of Medicine, Houston, TX, USA. lokeshwar.bhenderu@houstonmethodist.org., Taghlabi KM; Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA.; Center for Neural Systems Restoration, Houston Methodist Research Institute, Houston, TX, USA., Frazee KP; Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA.; School of Engineering, Texas A&M, College Station, TX, USA., Guerrero JR; Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA., Hogan MK; Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA.; Center for Neural Systems Restoration, Houston Methodist Research Institute, Houston, TX, USA.; Center for Neuroregeneration, Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA., Humes F; Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA.; Center for Neuroregeneration, Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA., Rostomily RC; Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA.; Center for Neuroregeneration, Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA., Horner PJ; Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA.; Center for Neural Systems Restoration, Houston Methodist Research Institute, Houston, TX, USA.; Center for Neuroregeneration, Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA., Faraji AH; Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA. ahfaraji@houstonmethodist.org.; Center for Neural Systems Restoration, Houston Methodist Research Institute, Houston, TX, USA. ahfaraji@houstonmethodist.org.; Center for Neuroregeneration, Department of Neurosurgery, Houston Methodist Research Institute, Houston, TX, USA. ahfaraji@houstonmethodist.org.
Jazyk: angličtina
Zdroj: Communications biology [Commun Biol] 2024 Jul 17; Vol. 7 (1), pp. 869. Date of Electronic Publication: 2024 Jul 17.
DOI: 10.1038/s42003-024-06404-1
Abstrakt: Electrokinetic convection-enhanced delivery (ECED) utilizes an external electric field to drive the delivery of molecules and bioactive substances to local regions of the brain through electroosmosis and electrophoresis, without the need for an applied pressure. We characterize the implementation of ECED to direct a neutrally charged fluorophore (3 kDa) from a doped biocompatible acrylic acid/acrylamide hydrogel placed on the cortical surface. We compare fluorophore infusion profiles using ECED (time = 30 min, current = 50 µA) and diffusion-only control trials, for ex vivo (N = 18) and in vivo (N = 12) experiments. The linear intensity profile of infusion to the brain is significantly higher in ECED compared to control trials, both for in vivo and ex vivo. The linear distance of infusion, area of infusion, and the displacement of peak fluorescence intensity along the direction of infusion in ECED trials compared to control trials are significantly larger for in vivo trials, but not for ex vivo trials. These results demonstrate the effectiveness of ECED to direct a solute from a surface hydrogel towards inside the brain parenchyma based predominantly on the electroosmotic vector.
(© 2024. The Author(s).)
Databáze: MEDLINE
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