Artemisia argyi volatile oil ameliorates allergic contact dermatitis via modulating TRPA1/CGRP signaling.

Autor: Yan T; School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan, 430074, China., Luo M; School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan, 430074, China., He J; School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan, 430074, China., Wang M; School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan, 430074, China., Ma Z; Qinghai Tibetan Medicine Research Institute, Qinghai Province Key Laboratory of Tibetan Medicine Research and Development, Xining, 810016, China., Zhao Z; Barnes-Jewish Hospital, St. Louis, MO, 63110, USA., Xiong H; School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan, 430074, China. Electronic address: xionghui0311020@163.com., Mei Z; School of Pharmaceutical Sciences, South-Central Minzu University, Wuhan, 430074, China; College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China. Electronic address: meizhinan@163.com.
Jazyk: angličtina
Zdroj: Journal of ethnopharmacology [J Ethnopharmacol] 2024 Nov 15; Vol. 334, pp. 118580. Date of Electronic Publication: 2024 Jul 15.
DOI: 10.1016/j.jep.2024.118580
Abstrakt: Ethnopharmacological Relevance: The leaves of Artemisia argyi Levl.et Vant. have a long history of being used to treat skin diseases such as pruritus and dermatitis in China, but the therapeutic effect on allergic contact dermatitis (ACD) is still unclear.
Aim of the Study: To investigate the effect and molecular mechanisms of the volatile oil of A. argyi leaves (abbreviated as 'AO') in the treatment of ACD.
Materials and Methods: The main components in AO were analyzed using GC-MS. The effect of AO on channel currents in hTRPA1-transfected HEK293T cells was studied by whole-cell patch clamp. Subsequently, chloroquine-evoked acute itch and squaraine dibutyl ester (SADBE)-induced ACD chronic itch model was established to evaluate the antipruritic effect through counting scratching behavior, and the anti-inflammatory effects on ACD mice were measured using histological analysis. Meanwhile, the changes of CGRP, the infiltration of nerve fibers and the recruitment of dendritic cells, the expression of Il-23 and Il-17 mRNA in skin lesions, the phosphorylation of ERK and p38 in dorsal root ganglion (DRG), were evaluated by molecular biological methods. Then the inhibitory effect of AO on AITC- or SADBE-activated TRPA1 channels in primary DRG neurons of C57BL/6, Trpa1 -/- or Trpv1 -/- mice was elucidated by Ca 2+ imaging and immunofluorescence.
Results: AO treatment inhibited the activation of TRPA1 in HEK293T cells and alleviated acute itch caused by chloroquine, but this effect was lacking in Trpa1 -/- mice. Furthermore, administration of AO attenuated scratching behavior in SADBE-induced ACD mice. AO also inhibited the increase of nerve fibers and recruitment of dendritic cells, and down-regulated the expression of CGRP and the levels of Il-23 and Il-17 mRNA. Meanwhile, AO reduced the expression of p-p38 and p-ERK in the lesioned skin and DRG of SADBE-induced ACD mice. Additionally, AO blocked the activation of TRPA1 channels and decreased the levels of CGRP, p-p38, and p-ERK in DRG neurons.
Conclusion: AO could inhibit TRPA1 channels in sensory neurons, thereby reducing the release of CGRP and exerting anti-pruritic and anti-inflammatory effect. These findings also provide a new strategy for exploring the role of A. argyi in treating ACD.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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