Phosphorous speciation in EPS extracted from Aerobic Granular Sludge.

Autor: Bahgat NT; Wetsus, European Centre Of Excellence for Sustainable Water Technology, Oostergoweg 7, 8911 MA, Leeuwarden, the Netherlands; Dept. Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, the Netherlands. Electronic address: t.m.s.m.bahgat@tudelft.nl., Wilfert P; Dept. Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, the Netherlands., Eustace SJ; Dept. Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, the Netherlands., Korving L; Wetsus, European Centre Of Excellence for Sustainable Water Technology, Oostergoweg 7, 8911 MA, Leeuwarden, the Netherlands., van Loosdrecht MCM; Dept. Biotechnology, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, the Netherlands; Center for Microbial Communities, Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark.
Jazyk: angličtina
Zdroj: Water research [Water Res] 2024 Sep 15; Vol. 262, pp. 122077. Date of Electronic Publication: 2024 Jul 11.
DOI: 10.1016/j.watres.2024.122077
Abstrakt: Wastewater treatment technologies opened the door for recovery of extracellular polymeric substances (EPS), presenting novel opportunities for use across diverse industrial sectors. Earlier studies showed that a significant amount of phosphorus (P) is recovered within extracted EPS. P recovered within the extracted EPS is an intrinsic part of the recovered material that potentially influences its properties. Understanding the P speciation in extracted EPS lays the foundation for leveraging the incorporated P in EPS to manipulate its properties and industrial applications. This study evaluated P speciation in EPS extracted from aerobic granular sludge (AGS). A fractionation lab protocol was established to consistently distinguish P species in extracted EPS liquid phase and polymer chains. 31 P nuclear magnetic resonance (NMR) spectroscopy was used as a complementary technique to provide additional information on P speciation and track changes in P species during the EPS extraction process. Findings showed the dominance of organic phosphorus and orthophosphates within EPS, besides other minor fractions. On average, 25% orthophosphates in the polymer liquid phase, 52% organic phosphorus (equal ratio of mono and diesters) covalently bound to the polymer chains, 16% non-apatite inorganic phosphorus (NAIP) precipitates mainly FeP and AlP, and 7% pyrophosphates (6% in the liquid phase and 1% attached to the polymer chains) were identified. Polyphosphates were detected in initial AGS but hydrolyzed to orthophosphates, pyrophosphates, and possibly organic P (forming new esters) during the EPS extraction process. The knowledge created in this study is a step towards the goal of EPS engineering, manipulating P chemistry along the extraction process and enriching certain P species in EPS based on target properties and industrial applications.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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