Mixotrophic growth of a ubiquitous marine diatom.

Autor: Kumar M; Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA., Tibocha-Bonilla JD; Bioinformatics and Systems Biology Graduate Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA., Füssy Z; Department of Parasitology, Faculty of Science, Charles University, BIOCEV, Vestec, Czech Republic., Lieng C; Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA., Schwenck SM; Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA., Levesque AV; Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA., Al-Bassam MM; Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA., Passi A; Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA., Neal M; Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA., Zuniga C; Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA., Kaiyom F; Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA., Espinoza JL; Department of Microbial and Environmental Genomics, J. Craig Venter Institute, 4120 Capricorn Way, La Jolla, CA 92037, USA., Lim H; Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA., Polson SW; Department of Computer and Information Sciences, University of Delaware, 18 Amstel Ave., Newark, DE 19716, USA.; Center for Bioinformatics and Computational Biology, University of Delaware, 590 Avenue 1743, Newark, DE 19713, USA., Allen LZ; Scripps Institution of Oceanography, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.; Department of Microbial and Environmental Genomics, J. Craig Venter Institute, 4120 Capricorn Way, La Jolla, CA 92037, USA., Zengler K; Department of Pediatrics, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.; Department of Bioengineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.; Center for Microbiome Innovation, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.; Program in Materials Science and Engineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
Jazyk: angličtina
Zdroj: Science advances [Sci Adv] 2024 Jul 19; Vol. 10 (29), pp. eado2623. Date of Electronic Publication: 2024 Jul 17.
DOI: 10.1126/sciadv.ado2623
Abstrakt: Diatoms are major players in the global carbon cycle, and their metabolism is affected by ocean conditions. Understanding the impact of changing inorganic nutrients in the oceans on diatoms is crucial, given the changes in global carbon dioxide levels. Here, we present a genome-scale metabolic model ( i MK1961) for Cylindrotheca closterium , an in silico resource to understand uncharacterized metabolic functions in this ubiquitous diatom. i MK1961 represents the largest diatom metabolic model to date, comprising 1961 open reading frames and 6718 reactions. With i MK1961, we identified the metabolic response signature to cope with drastic changes in growth conditions. Comparing model predictions with Tara Oceans transcriptomics data unraveled C. closterium 's metabolism in situ. Unexpectedly, the diatom only grows photoautotrophically in 21% of the sunlit ocean samples, while the majority of the samples indicate a mixotrophic (71%) or, in some cases, even a heterotrophic (8%) lifestyle in the light. Our findings highlight C. closterium' s metabolic flexibility and its potential role in global carbon cycling.
Databáze: MEDLINE