An integrative approach to the study of Kempnyia Klapálek, 1914 (Plecoptera: Perlidae) from Brazil: Support for the description of four new species and a basis for future studies.

Autor: de Almeida LH; Laboratório de Biologia Aquática, Faculdade de Ciências e Letras de Assis, Universidade Estadual Paulista, Assis, São Paulo, Brazil., Gonçalves MC; PPG em Entomologia, Universidade Federal de Viçosa (UFV), Viçosa, Minas Gerais, Brazil., da Conceição Bispo P; Laboratório de Biologia Aquática, Faculdade de Ciências e Letras de Assis, Universidade Estadual Paulista, Assis, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2024 Jul 17; Vol. 19 (7), pp. e0305824. Date of Electronic Publication: 2024 Jul 17 (Print Publication: 2024).
DOI: 10.1371/journal.pone.0305824
Abstrakt: Kempnyia (Plecoptera: Perlidae) is an endemic genus of Brazilian stoneflies that has 36 valid species and is distributed primarily in the Atlantic Forest and the mountainous areas of Central Brazil, particularly in Goiás and Tocantins states. Despite being the Brazilian genus with the most DNA sequences available on GenBank, integrative studies on the genus began only recently, in 2014. In this context, herein we studied the morphology and molecular data of Kempnyia specimens deposited in the Aquatic Biology Laboratory (UNESP, Assis) and the Entomology Museum of the Federal University of Viçosa (UFVB, Viçosa) collections. For the integrative approach adopted, in addition to studying the specimens morphologically, we used sequences of the COI mitochondrial gene combined with the following species delimitation methods: Automatic Barcode Gap Discovery (ABGD), both primary (ABGDp) and recursive (ABGDr) partitions; Assemble Species by Automatic Partitioning (ASAP); Poisson Tree Processes (PTP) and the Bayesian implementation of the Poisson Tree Processes (bPTP). As a result, we provided 28 new COI sequences of 21 species and support the description of four new species, namely, K. guarani sp. nov., K. tupiniquim sp. nov., K. una sp. nov., and K. zwickii sp. nov., consequently increasing the known diversity of the genus to 40 species. We also discuss the morphological variations observed in other species of the genus and provide several new geographic records. Therefore, our study brings new insights into the values of intra- and interspecific molecular divergence within Kempnyia, serving as a basis for new studies.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 de Almeida et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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