Rifabutin loaded inhalable β-glucan microparticle based drug delivery system for pulmonary TB.

Autor: Ahmad F; IIRC-3 Immunobiochemistry Lab, Department of Biosciences, Integral University, Lucknow, UP, 226026, India.; Department of Clinical Immunology & Rheumatology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, UP, 226014, India., Ahmad S; Department of Biochemistry, Dr. Ram Manohar Lohia Avadh University, Faizabad, UP, 224001, India., Upadhyay TK; Department of Life Sciences, Parul Institute of Applied Sciences & Research and Development Cell, Parul University, Vadodara, Gujarat, 391760, India., Singh S; Pharmaceutics and Pharmacokinetics Division, CSIR-CDRI, Lucknow, UP, 226201, India., Khubaib M; IIRC-3 Immunobiochemistry Lab, Department of Biosciences, Integral University, Lucknow, UP, 226026, India., Singh J; Inhalation Toxicology Facility, CSIR-Indian Institute of Toxicology Research, Lucknow, UP, 226008, India., Saeed M; Department of Biology, College of Sciences, University of Hail, 34464, Hail, Saudi Arabia., Ahmad I; Department of Clinical Laboratory Sciences, College of Applied Medical Science, King Khalid University, Abha, Saudi Arabia., Al-Keridis LA; Department of Biology, Faculty of Science, Princess Nourah bint Abdulrahman University, P.O.Box 84428, 11671, Riyadh, Saudi Arabia., Sharma R; Department of Life Sciences and Biotechnology, CSJM University, Kanpur, UP, 228024, India. roleesharma@csjmu.ac.in.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 Jul 16; Vol. 14 (1), pp. 16437. Date of Electronic Publication: 2024 Jul 16.
DOI: 10.1038/s41598-024-66634-5
Abstrakt: Inhalable microparticle-based anti TB drug delivery systems are being investigated extensively for Tuberculosis [TB] treatment as they offer efficient and deep lung deposition with several advantages over conventional routes. It can reduce the drug dose, treatment duration and toxic effects and optimize the drug bioavailability. Yeast derived β-glucan is a β-[1-3/1-6] linked biocompatible polymer and used as carrier for various biomolecules. Due to presence of glucan chains, particulate glucans act as PAMP and thereby gets internalized via receptor mediated phagocytosis by the macrophages. In this study, β-glucan microparticles were prepared by adding l-leucine as excipient, and exhibited 70% drug [Rifabutin] loading efficiency. Further, the sizing and SEM data of particles revealed a size of 2-4 µm with spherical dimensions. The FTIR and HPLC data confirmed the β-glucan composition and drug encapsulations efficiency of the particles. The mass median aerodynamic diameter [MMAD] and geometric standard deviation [GSD] data indicated that these particles are inhalable in nature and have better thermal stability as per DSC thermogram. These particles were found to be non-toxic upto a concentration of 80 µg/ml and were found to be readily phagocytosed by human macrophage cells in-vitro as well as in-vivo by lung alveolar macrophage. This study provides a framework for future design of inhalable β-glucan particle based host-directed drug delivery system against pulmonary TB.
(© 2024. The Author(s).)
Databáze: MEDLINE
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