Immunoaffinity Intact-Mass Spectrometry for the Detection of Endogenous Concentrations of the Acetylated Protein Tumor Biomarker Neuron Specific Enolase.

Autor: van den Wildenberg SAH; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands.; Clinical Laboratory, Catharina Hospital, 5623 EJ Eindhoven, The Netherlands.; Expert Center Clinical Chemistry Eindhoven, 5612 AZ Eindhoven, The Netherlands., Genet SAAM; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands.; Clinical Laboratory, Catharina Hospital, 5623 EJ Eindhoven, The Netherlands.; Expert Center Clinical Chemistry Eindhoven, 5612 AZ Eindhoven, The Netherlands., Broeren MAC; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands.; Expert Center Clinical Chemistry Eindhoven, 5612 AZ Eindhoven, The Netherlands.; Clinical Laboratory, Máxima Medical Center, 5504 DB Veldhoven, The Netherlands., van Dongen JLJ; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands.; Expert Center Clinical Chemistry Eindhoven, 5612 AZ Eindhoven, The Netherlands., Brunsveld L; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands.; Expert Center Clinical Chemistry Eindhoven, 5612 AZ Eindhoven, The Netherlands., Scharnhorst V; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands.; Clinical Laboratory, Catharina Hospital, 5623 EJ Eindhoven, The Netherlands.; Expert Center Clinical Chemistry Eindhoven, 5612 AZ Eindhoven, The Netherlands., van de Kerkhof D; Laboratory of Chemical Biology, Department of Biomedical Engineering, Eindhoven University of Technology, 5612 AZ Eindhoven, The Netherlands.; Clinical Laboratory, Catharina Hospital, 5623 EJ Eindhoven, The Netherlands.; Expert Center Clinical Chemistry Eindhoven, 5612 AZ Eindhoven, The Netherlands.
Jazyk: angličtina
Zdroj: Journal of proteome research [J Proteome Res] 2024 Aug 02; Vol. 23 (8), pp. 3726-3730. Date of Electronic Publication: 2024 Jul 16.
DOI: 10.1021/acs.jproteome.4c00391
Abstrakt: Intact-mass spectrometry has huge potential for clinical application, as it enables both quantitative and qualitative analysis of intact proteins and possibly unlocks additional pathophysiological information via, e.g., detection of specific post-translational modifications (PTMs). Such valuable and clinically useful selectivity is typically lost during conventional bottom-up mass spectrometry. We demonstrate an innovative immunoprecipitation protein enrichment assay coupled to ultrahigh performance liquid chromatography quadrupole time-of-flight high resolution mass spectrometry (UPLC-QToF-HRMS) for the fast and simple identification of the protein tumor marker Neuron Specific Enolase Gamma (NSEγ) at low endogenous concentrations in human serum. Additionally, using the combination of immunoaffinity purification with intact mass spectrometry, the presence of NSEγ in an acetylated form in human serum was detected. This highlights the unique potential of immunoaffinity intact mass spectrometry in clinical diagnostics.
Databáze: MEDLINE
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