Mangiferin potentiates the activity of antifungal agents against fluconazole-resistant Candida spp.

Autor: Ferreira TL; Department of Clinical & Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza 60.430-370, Ceará, Brazil.; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil., Leitão AC; Department of Clinical & Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza 60.430-370, Ceará, Brazil.; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil., da Silva LJ; Department of Clinical & Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza 60.430-370, Ceará, Brazil.; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil., do Amaral Valente Sá LG; Department of Clinical & Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza 60.430-370, Ceará, Brazil.; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil.; Christus University Center, Fortaleza 60.190-180, Ceará,Brazil., de Farias Cabral VP; Department of Clinical & Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza 60.430-370, Ceará, Brazil.; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil., Rodrigues DS; Department of Clinical & Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza 60.430-370, Ceará, Brazil.; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil., Barbosa SA; Department of Clinical & Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza 60.430-370, Ceará, Brazil.; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil., de Andrade Neto JB; Department of Clinical & Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza 60.430-370, Ceará, Brazil.; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil.; Christus University Center, Fortaleza 60.190-180, Ceará,Brazil., Barbosa AD; Department of Clinical & Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza 60.430-370, Ceará, Brazil.; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil., Almeida Moreira LE; Department of Clinical & Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza 60.430-370, Ceará, Brazil.; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil., França Rios ME; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil., Cavalcanti BC; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil., de Moraes MO; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil., Nobre Júnior HV; Department of Clinical & Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza 60.430-370, Ceará, Brazil.; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil., da Silva CR; Department of Clinical & Toxicological Analysis, Faculty of Pharmacy, Laboratory of Bioprospection in Antimicrobial Molecules (LABIMAN), Federal University of Ceará, Fortaleza 60.430-370, Ceará, Brazil.; Drug Research and Development Center, Federal University of Ceará, Fortaleza 60.430-275, Ceará, Brazil.
Jazyk: angličtina
Zdroj: Future microbiology [Future Microbiol] 2024 Sep; Vol. 19 (13), pp. 1157-1170. Date of Electronic Publication: 2024 Jul 16.
DOI: 10.1080/17460913.2024.2366627
Abstrakt: Aim: To evaluate the antifungal activity of mangiferin against Candida spp. resistant to fluconazole. Materials & methods: The antifungal activity of mangiferin was assessed using broth microdilution and its interaction with azoles and amphotericin B was evaluated by checkerboard. The activity of mangiferin against Candida spp. biofilms was assessed using the MTT colorimetric assay and its possible mechanism of action was evaluated using flow cytometry. Results: Mangiferin showed activity against Candida albicans, Candida tropicalis and Candida parapsilosis resistant to fluconazole and showed synergism with azoles and amphotericin B. Mangiferin increased the activity of antifungals against Candida biofilms and caused depolarization of the mitochondrial membrane and externalization of phosphatidylserine, suggesting apoptosis. Conclusion: mangiferin combined with antifungals has potential against Candida spp.
Databáze: MEDLINE