Comparative genomics and stable isotope analysis reveal the saprotrophic-pathogenic lifestyle of a neotropical fungus.

Autor:	Ribeiro Tomé LM; Department of Microbiology, Molecular and Computational Biology of Fungi Laboratory, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Quintanilha-Peixoto G; Department of Microbiology, Molecular and Computational Biology of Fungi Laboratory, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil., Costa-Rezende DH; Departamento de Ciências Biológicas, Programa de Pós-graduação em Botânica, Universidade Estadual de Feira de Santana, Feira de Santana, Brazil., Salvador-Montoya CA; MIND.Funga (Monitoring and Inventorying Neotropical Diversity of Fungi) - MICOLAB, Universidade Federal de Santa Catarina, Florianópolis, Brazil.; Fundación Miguel Lillo, Instituto Criptogámico-Sección Micología, San Miguel de Tucumán, Argentina.; Organización Juvenil 'Hongos Perú', Cusco, Santiago, Peru., Cardoso D; Instituto de Pesquisas Jardim Botânico do Rio de Janeiro (JBRJ), Rio de Janeiro, Brazil.; Instituto de Biologia, Universidade Federal da Bahia, Salvador, Brazil., S Araújo D; Program in Bioinformatics, Loyola University Chicago, Chicago, Illinois, USA., Freitas JM; Departamento de Ecologia, Universidade de Brasília, Brasília, Brazil., Bielefeld Nardoto G; Departamento de Ecologia, Universidade de Brasília, Brasília, Brazil., Alves-Silva G; MIND.Funga (Monitoring and Inventorying Neotropical Diversity of Fungi) - MICOLAB, Universidade Federal de Santa Catarina, Florianópolis, Brazil., Drechsler-Santos ER; MIND.Funga (Monitoring and Inventorying Neotropical Diversity of Fungi) - MICOLAB, Universidade Federal de Santa Catarina, Florianópolis, Brazil., Góes-Neto A; Department of Microbiology, Molecular and Computational Biology of Fungi Laboratory, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Jazyk:	angličtina
Zdroj:	MBio [mBio] 2024 Aug 14; Vol. 15 (8), pp. e0142324. Date of Electronic Publication: 2024 Jul 16.
DOI:	10.1128/mbio.01423-24
Abstrakt:	In terrestrial forested ecosystems, fungi may interact with trees in at least three distinct ways: (i) associated with roots as symbionts; (ii) as pathogens in roots, trunks, leaves, flowers, and fruits; or (iii) decomposing dead tree tissues on soil or even on dead tissues in living trees. Distinguishing the latter two nutrition modes is rather difficult in Hymenochaetaceae (Basidiomycota) species. Herein, we have used an integrative approach of comparative genomics, stable isotopes, host tree association, and bioclimatic data to investigate the lifestyle ecology of the scarcely known neotropical genus Phellinotus , focusing on the unique species Phellinotus piptadeniae . This species is strongly associated with living Piptadenia gonoacantha (Fabaceae) trees in the Atlantic Forest domain on a relatively high precipitation gradient. Phylogenomics resolved P. piptadeniae in a clade that also includes both plant pathogens and typical wood saprotrophs. Furthermore, both genome-predicted Carbohydrate-Active Enzymes (CAZy) and stable isotopes (δ 13 C and δ 15 N) revealed a rather flexible lifestyle for the species. Altogether, our findings suggest that P. piptadeniae has been undergoing a pathotrophic specialization in a particular tree species while maintaining all the metabolic repertoire of a wood saprothroph. Importance: This is the first genomic description for Phellinotus piptadeniae . This basidiomycete is found across a broad range of climates and ecosystems in South America, including regions threatened by extensive agriculture. This fungus is also relevant considering its pathotrophic-saprotrophic association with Piptadenia goanocantha , which we began to understand with these new results that locate this species among biotrophic and necrotrophic fungi. Competing Interests: The authors declare no conflict of interest.
Databáze:	MEDLINE
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