Three-dimensional lung parenchyma model for studies of Aspergillus fumigatus infection and antifungal treatment.

Autor: Fortes BN; Institute of Biomedical Sciences, University of São Paulo, Prof. Lineu Prestes Avenue, 1374, 05508-000, São Paulo, Brazil., Wirth F; Institute of Biomedical Sciences, University of São Paulo, Prof. Lineu Prestes Avenue, 1374, 05508-000, São Paulo, Brazil., Dos Santos AM; School of Pharmaceutical Sciences, São Paulo State University - Jaú Highway, Km 1, 14800-903, Araraquara, Brazil., Chorilli M; School of Pharmaceutical Sciences, São Paulo State University - Jaú Highway, Km 1, 14800-903, Araraquara, Brazil., Freitas VM; Institute of Biomedical Sciences, University of São Paulo, Prof. Lineu Prestes Avenue, 1374, 05508-000, São Paulo, Brazil., Farias J; School of Arts, Sciences & Humanities, University of São Paulo, Arlindo Bettio Avenue, 1000, 03828-000, São Paulo, Brazil., Chambergo FS; School of Arts, Sciences & Humanities, University of São Paulo, Arlindo Bettio Avenue, 1000, 03828-000, São Paulo, Brazil., Nunes C Dantas VA; School of Arts, Sciences & Humanities, University of São Paulo, Arlindo Bettio Avenue, 1000, 03828-000, São Paulo, Brazil., Ishida K; Institute of Biomedical Sciences, University of São Paulo, Prof. Lineu Prestes Avenue, 1374, 05508-000, São Paulo, Brazil.
Jazyk: angličtina
Zdroj: Future microbiology [Future Microbiol] 2024; Vol. 19 (14), pp. 1203-1216. Date of Electronic Publication: 2024 Jul 16.
DOI: 10.1080/17460913.2024.2371926
Abstrakt: Aim: This work aims to standardize the three-dimensional hydroxyethyl-alginate-gelatin (HAG) scaffold as a model to evaluate Aspergillus fumigatus biofilm and antifungal treatments. Methods: The scaffold was characterized by physical, rheological and microscopic analyses; the antibiofilm action was evaluated by determination of cfu and metabolic activity. Results: The scaffold was non-toxic showing stability in aqueous media, swelling capacity, elasticity and had homogeneously distributed pores averaging 190 μm. The A. fumigatus biofilm established itself very well on the scaffold and treatment with amphotericin B and voriconazole reduced viable cells and metabolic activity. Conclusion: The HAG scaffold proved to be a model to mimic lung parenchyma, suitable for establishing a 3D biofilm culture of A. fumigatus and evaluating the efficacy of antifungals.
Databáze: MEDLINE
Externí odkaz: