| Autor: |
Ngo MT; Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.; Department of Medicinal Chemistry and Pharmacology, University of Science and Technology, Daejeon 34113, Republic of Korea., Han JW; Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea., Nguyen MV; Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.; Department of Medicinal Chemistry and Pharmacology, University of Science and Technology, Daejeon 34113, Republic of Korea., Choi Y; Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea., Kim B; Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.; Department of Medicinal Chemistry and Pharmacology, University of Science and Technology, Daejeon 34113, Republic of Korea., Gho ES; Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.; Department of Medicinal Chemistry and Pharmacology, University of Science and Technology, Daejeon 34113, Republic of Korea., Kim H; Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.; Department of Medicinal Chemistry and Pharmacology, University of Science and Technology, Daejeon 34113, Republic of Korea., Choi GJ; Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology, Daejeon 34114, Republic of Korea.; Department of Medicinal Chemistry and Pharmacology, University of Science and Technology, Daejeon 34113, Republic of Korea. |
| Abstrakt: |
In our screening program for natural products that are effective in controlling plant diseases, we found that the culture filtrate of Paraconiothyrium sporulosum SFC20160907-M11 effectively suppressed the development of tomato late blight disease caused by Phytophthora infestans . Using a bioassay-guided fractionation of antioomycete activity, 12 active compounds ( 1 - 12 ) were obtained from an ethyl acetate extract of the culture filtrate. Chemical structures of five new compounds 1 - 5 were determined by the extensive analyses of nuclear magnetic resonance (NMR), high resolution mass spectrometry (HRMS), and circular dichroism (CD) data. Interestingly, mycosporulonol ( 1 ) and botrallin ( 8 ) completely inhibited the growth of P. infestans at concentrations of 8 and 16 μg/mL, respectively. Furthermore, the spray treatment of 1 and 8 (500 μg/mL) successfully protected tomato seedlings against P. infestans with disease control values of 92%. Taken together, these results suggest that the culture filtrates of P. sporulosum SFC20160907-M11 and their bioactive metabolites can be used as new antioomycete agents for Phytophthora late blight control. |
