Mechanoactivation of Single Stem Cells in Microgels Using a 3D-Printed Stimulation Device.

Autor: İyisan N; Microrobotic Bioengineering Lab (MRBL), School of Computation, Information, and Technology, Department of Electrical Engineering, Technical University of Munich (TUM), Hans-Piloty-Straße 1, 85748, Garching, Germany.; Munich Institute of Robotics and Machine Intelligence, Technical University of Munich, Georg-Brauchle-Ring 60, 80992, München, Germany.; Munich Institute of Biomedical Engineering, Technical University of Munich, Boltzmannstraße 11, 85748, Garching, Germany., Hausdörfer O; Microrobotic Bioengineering Lab (MRBL), School of Computation, Information, and Technology, Department of Electrical Engineering, Technical University of Munich (TUM), Hans-Piloty-Straße 1, 85748, Garching, Germany., Wang C; Microrobotic Bioengineering Lab (MRBL), School of Computation, Information, and Technology, Department of Electrical Engineering, Technical University of Munich (TUM), Hans-Piloty-Straße 1, 85748, Garching, Germany.; Munich Institute of Robotics and Machine Intelligence, Technical University of Munich, Georg-Brauchle-Ring 60, 80992, München, Germany.; Munich Institute of Biomedical Engineering, Technical University of Munich, Boltzmannstraße 11, 85748, Garching, Germany., Hiendlmeier L; Munich Institute of Biomedical Engineering, Technical University of Munich, Boltzmannstraße 11, 85748, Garching, Germany.; Neuroelectronics, School of Computation, Information, and Technology, Department of Electrical Engineering, Department of Electrical Engineering, Technical University of Munich (TUM), 85748, Garching, Germany., Harder P; Microrobotic Bioengineering Lab (MRBL), School of Computation, Information, and Technology, Department of Electrical Engineering, Technical University of Munich (TUM), Hans-Piloty-Straße 1, 85748, Garching, Germany.; Munich Institute of Robotics and Machine Intelligence, Technical University of Munich, Georg-Brauchle-Ring 60, 80992, München, Germany.; Munich Institute of Biomedical Engineering, Technical University of Munich, Boltzmannstraße 11, 85748, Garching, Germany., Wolfrum B; Munich Institute of Biomedical Engineering, Technical University of Munich, Boltzmannstraße 11, 85748, Garching, Germany.; Neuroelectronics, School of Computation, Information, and Technology, Department of Electrical Engineering, Department of Electrical Engineering, Technical University of Munich (TUM), 85748, Garching, Germany., Özkale B; Microrobotic Bioengineering Lab (MRBL), School of Computation, Information, and Technology, Department of Electrical Engineering, Technical University of Munich (TUM), Hans-Piloty-Straße 1, 85748, Garching, Germany.; Munich Institute of Robotics and Machine Intelligence, Technical University of Munich, Georg-Brauchle-Ring 60, 80992, München, Germany.; Munich Institute of Biomedical Engineering, Technical University of Munich, Boltzmannstraße 11, 85748, Garching, Germany.
Jazyk: angličtina
Zdroj: Small methods [Small Methods] 2024 Jul 16, pp. e2400272. Date of Electronic Publication: 2024 Jul 16.
DOI: 10.1002/smtd.202400272
Abstrakt: In this study, the novel 3D-printed pressure chamber for encapsulated single-cell stimulation (3D-PRESS) platform is introduced for the mechanical stimulation of single stem cells in 3D microgels. The custom-designed 3D-PRESS, allows precise pressure application up to 400 kPa at the single-cell level. Microfluidics is employed to encapsulate single mesenchymal stem cells within ionically cross-linked alginate microgels with cell adhesion RGD peptides. Rigorous testing affirms the leak-proof performance of the 3D-PRESS device up to 400 kPa, which is fully biocompatible. 3D-PRESS is implemented on mesenchymal stem cells for mechanotransduction studies, by specifically targeting intracellular calcium signaling and the nuclear translocation of a mechanically sensitive transcription factor. Applying 200 kPa pressure on individually encapsulated stem cells reveals heightened calcium signaling in 3D microgels compared to conventional 2D culture. Similarly, Yes-associated protein (YAP) translocation into the nucleus occurs at 200 kPa in 3D microgels with cell-binding RGD peptides unveiling the involvement of integrin-mediated mechanotransduction in singly encapsulated stem cells in 3D microgels. Combining live-cell imaging with precise mechanical control, the 3D-PRESS platform emerges as a versatile tool for exploring cellular responses to pressure stimuli, applicable to various cell types, providing novel insights into single-cell mechanobiology.
(© 2024 The Author(s). Small Methods published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
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