Therapeutic Efficacy of Selenium Pre-treatment in Mitigating Cadmium-Induced Cardiotoxicity in Zebrafish (Danio rerio).

Autor:	Heuer RM; University of Miami., Falagan-Lotsch P; Auburn University., Okutsu J; University of Miami., Deperalto M; University of Miami., Koop RR; University of Miami., Umeh OG; University of Miami., Guevara GA; University of Miami., Noor MI; University of Miami., Covington MA; University of Miami., Shelton DS; University of Miami.
Jazyk:	angličtina
Zdroj:	Research square [Res Sq] 2024 Jul 03. Date of Electronic Publication: 2024 Jul 03.
DOI:	10.21203/rs.3.rs-4583781/v1
Abstrakt:	Cardiovascular diseases are a rampant public health threat. Environmental contaminants, such as Cadmium (Cd), a toxic metal, have been linked to increased risk for cardiovascular diseases. Given that human exposure to Cd is increasing overtime, there is a need to develop new therapies to ameliorate Cd toxicity. Selenium (Se), an essential trace element, has been proposed to rescue the effects of Cd toxicity, with mixed effects. Se's narrow therapeutic window necessitates precise dosing to avoid toxicity. Here, we assessed the effects of various waterborne Cd and Se concentrations and sequences on cardiac function using zebrafish ( Danio rerio ). We showed that Cd induced pericardial edemas and modified heart rates in a concentration-dependent manner. To identify the therapeutic range of Se for Cd-induced cardiotoxicity, zebrafish embryos were treated with 0, 10, 50, 100, 150, or 200 μg/L Se for 1-4 days prior to exposure to Cd at 2.5, and 5 μg/L. We found that a 50 μg/L Se pre-treatment prior to Cd at 2.5 μg/L, but not at 5 μg/L, reduced the prevalence of pericardial edemas and ameliorated Cd-induced bradycardia in zebrafish. Embryos exposed to 10 and 50 μg/L of Se showed typical heart morphology, whereas other Se-exposed and Se-deficient fish presented pericardial edemas. Longer Se pre-treatment durations led to fewer incidences of pericardial edemas. Overall, this study highlights the importance of optimizing Se concentration and pre-treatment periods to harness its protective effects against Cd-induced cardiotoxicity. These findings provide insights into potential therapeutic strategies for reducing Cd-related cardiovascular damage in humans. Competing Interests: Declarations The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Delia S. Shelton reports financial support was provided by National Institutes of Health National Institute of Environmental Health Sciences.
Databáze:	MEDLINE
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