Conformational transitions in redissolved silk fibroin films and application for printable self-powered multistate resistive memory biomaterials.

Autor: Libera V; Dipartimento di Fisica e Geologia, Università degli Studi di Perugia Via A. Pascoli 06123 Perugia Italy., Malaspina R; Dipartimento di Fisica e Geologia, Università degli Studi di Perugia Via A. Pascoli 06123 Perugia Italy., Bittolo Bon S; Dipartimento di Fisica e Geologia, Università degli Studi di Perugia Via A. Pascoli 06123 Perugia Italy., Cardinali MA; Department of Chemistry, Biology and Biotechnology, University of Perugia Via Elce di Sotto 8 06123 Perugia Italy., Chiesa I; Department of Ingegneria dell'Informazione, Research Center E. Piaggio, University of Pisa Largo Lucio Lazzarino 1 Pisa 56122 Italy., De Maria C; Department of Ingegneria dell'Informazione, Research Center E. Piaggio, University of Pisa Largo Lucio Lazzarino 1 Pisa 56122 Italy., Paciaroni A; Dipartimento di Fisica e Geologia, Università degli Studi di Perugia Via A. Pascoli 06123 Perugia Italy., Petrillo C; Dipartimento di Fisica e Geologia, Università degli Studi di Perugia Via A. Pascoli 06123 Perugia Italy., Comez L; CNR-IOM - Istituto Officina dei Materiali, National Research Council of Italy Via Alessandro Pascoli 06123 Perugia Italy., Sassi P; Department of Chemistry, Biology and Biotechnology, University of Perugia Via Elce di Sotto 8 06123 Perugia Italy., Valentini L; Civil and Environmental Engineering Department, INSTM Research Unit, University of Perugia Strada di Pentima 8 05100 Terni Italy luca.valentini@unipg.it.
Jazyk: angličtina
Zdroj: RSC advances [RSC Adv] 2024 Jul 15; Vol. 14 (31), pp. 22393-22402. Date of Electronic Publication: 2024 Jul 15 (Print Publication: 2024).
DOI: 10.1039/d4ra02830a
Abstrakt: 3D printing of water stable proteins with elastic properties offers a broad range of applications including self-powered biomedical devices driven by piezoelectric biomaterials. Here, we present a study on water-soluble silk fibroin (SF) films. These films were prepared by mixing degummed silk fibers and calcium chloride (CaCl 2 ) in formic acid, resulting in a silk I-like conformation, which was then converted into silk II by redissolving in phosphate buffer (PBS). Circular dichroism, Raman and infrared (IR) spectroscopies were used to investigate the transitions of secondary structure in silk I and silk II as the pH of the solvent and the sonication time were changed. We showed that a solvent with low pH ( e.g. 4) maintains the silk I β-turn structure; in contrast solvent with higher pH ( e.g. 7.4) promotes β-sheet features of silk II. Ultrasonic treatment facilitates the transition to water stable silk II only for the SF redissolved in PBS. SF from pH 7.4 solution has been printed using extrusion-based 3D printing. A self-powered memristor was realized, comprising an SF-based electric generator and an SF 3D-printed memristive unit connected in series. By exploiting the piezoelectric properties of silk II with higher β-sheet content and Ca 2+ ion transport phenomena, the application of an input voltage driven by a SF generator to SF 3D printed holey structures induces a variation from an initial low resistance state (LRS) to a high resistance state (HRS) that recovers in a few minutes, mimicking the transient memory, also known as short-term memory. Thanks to this holistic approach, these findings can contribute to the development of self-powered neuromorphic networks based on biomaterials with memory capabilities.
Competing Interests: There are no conflicts to declare.
(This journal is © The Royal Society of Chemistry.)
Databáze: MEDLINE
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