Biomechanical and biochemical changes in murine skin during development and aging.

Autor: Martyts A; Institute for Mechanical Systems, Department of Mechanical and Process Engineering, ETH Zürich, 8092 Zürich, Switzerland., Sachs D; Institute for Mechanical Systems, Department of Mechanical and Process Engineering, ETH Zürich, 8092 Zürich, Switzerland., Hiebert P; Institute of Molecular Health Sciences, Department of Biology, ETH Zürich, 8093 Zürich, Switzerland., Junker H; Institute for Mechanical Systems, Department of Mechanical and Process Engineering, ETH Zürich, 8092 Zürich, Switzerland., Robmann S; Institute for Mechanical Systems, Department of Mechanical and Process Engineering, ETH Zürich, 8092 Zürich, Switzerland., Hopf R; Institute for Mechanical Systems, Department of Mechanical and Process Engineering, ETH Zürich, 8092 Zürich, Switzerland., Steenbock H; Institute of Virology and Cell Biology, University of Lübeck, 23562 Lübeck, Germany., Brinckmann J; Institute of Virology and Cell Biology, University of Lübeck, 23562 Lübeck, Germany; Department of Dermatology, University of Lübeck, 23562 Lübeck, Germany., Werner S; Institute of Molecular Health Sciences, Department of Biology, ETH Zürich, 8093 Zürich, Switzerland., Giampietro C; Institute for Mechanical Systems, Department of Mechanical and Process Engineering, ETH Zürich, 8092 Zürich, Switzerland; Empa, Swiss Federal Laboratories for Materials Science and Technology, 8600 Dübendorf, Switzerland., Mazza E; Institute for Mechanical Systems, Department of Mechanical and Process Engineering, ETH Zürich, 8092 Zürich, Switzerland; Empa, Swiss Federal Laboratories for Materials Science and Technology, 8600 Dübendorf, Switzerland. Electronic address: mazza@imes.mavt.ethz.ch.
Jazyk: angličtina
Zdroj: Acta biomaterialia [Acta Biomater] 2024 Sep 15; Vol. 186, pp. 316-329. Date of Electronic Publication: 2024 Jul 14.
DOI: 10.1016/j.actbio.2024.07.013
Abstrakt: Aging leads to biochemical and biomechanical changes in skin, with biological and functional consequences. Despite extensive literature on skin aging, there is a lack of studies which investigate the maturation of the tissue and connect the microscopic changes in the skin to its macroscopic biomechanical behavior as it evolves over time. The present work addresses this knowledge gap using multiscale characterization of skin in a murine model considering newborn, adult and aged mice. Monotonic uniaxial loading, tension relaxation with change of bath, and loading to failure tests were performed on murine skin samples from different age groups, complemented by inflation experiments and atomic force microscopy indentation measurements. In parallel, skin samples were characterized using histological and biochemical techniques to assess tissue morphology, collagen organization, as well as collagen content and cross-linking. We show that 1-week-old skin differs across nearly all measured parameters from adult skin, showing reduced strain stiffening and tensile strength, a thinner dermis, lower collagen content and altered crosslinking patterns. Surprisingly, adult and aged skin were similar across most biomechanical parameters in the physiologic loading range, while aged skin had lower tensile strength and lower stiffening behavior at large force values. This correlates with altered collagen content and cross-links. Based on a computational model, differences in mechanocoupled stimuli in the skin of the different age groups were calculated, pointing to a potential biological significance of the age-induced biomechanical changes in regulating the local biophysical environment of dermal cells. STATEMENT OF SIGNIFICANCE: Skin microstructure and the emerging mechanical properties change with age, leading to biological, functional and health-related consequences. Despite extensive literature on skin aging, only very limited quantitative data are available on microstructural changes and the corresponding macroscopic biomechanical behavior as they evolve over time. This work provides a wide-range multiscale mechanical characterization of skin of newborn, adult and aged mice, and quantifies microstructural correlations in tissue morphology, collagen content, organization and cross-linking. Remarkably, aged skin retained normal hydration and normal biomechanical function in the physiological loading range but showed significantly reduced properties at super-physiological loading. Our data show that age-related microstructural differences have a profound effect not only on tissue-level properties but also on the cell-level biophysical environment.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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