Comparison of galactomannan lateral flow assay and enzyme immunoassay to identify Aspergillus spp. in bronchoalveolar lavage fluid.

Autor:	Martins SC; Universidade Estadual de Campinas (Unicamp) - Hospital das Clínicas, Faculdade de Ciências Médicas, Laboratório de Epidemiologia Molecular e Doenças Infecciosas (LEMDI), Cidade Universitária, Campinas, SP, Brazil., Tararam CA; Universidade Estadual de Campinas (Unicamp) - Hospital das Clínicas, Faculdade de Ciências Médicas, Laboratório de Epidemiologia Molecular e Doenças Infecciosas (LEMDI), Cidade Universitária, Campinas, SP, Brazil., Levy LO; Universidade Estadual de Campinas (Unicamp) - Hospital das Clínicas, Faculdade de Ciências Médicas, Laboratório de Epidemiologia Molecular e Doenças Infecciosas (LEMDI), Cidade Universitária, Campinas, SP, Brazil., Arai T; Chiba University, Division of Clinical Research, Medical Mycology Research Center (MMRC), Inohana, Chuo-ku, Chiba-shi, Chiba, Japan., Watanabe A; Chiba University, Division of Clinical Research, Medical Mycology Research Center (MMRC), Inohana, Chuo-ku, Chiba-shi, Chiba, Japan., Moretti ML; Universidade Estadual de Campinas (Unicamp) - Hospital das Clínicas, Faculdade de Ciências Médicas, Laboratório de Epidemiologia Molecular e Doenças Infecciosas (LEMDI), Cidade Universitária, Campinas, SP, Brazil., Trabasso P; Universidade Estadual de Campinas (Unicamp) - Hospital das Clínicas, Faculdade de Ciências Médicas, Laboratório de Epidemiologia Molecular e Doenças Infecciosas (LEMDI), Cidade Universitária, Campinas, SP, Brazil. Electronic address: trabasso@unicamp.br.
Jazyk:	angličtina
Zdroj:	The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases [Braz J Infect Dis] 2024 Jul-Aug; Vol. 28 (4), pp. 103838. Date of Electronic Publication: 2024 Jul 14.
DOI:	10.1016/j.bjid.2024.103838
Abstrakt:	Aspergillus species can colonize and infect immunocompetent and immunocompromised hosts. Conventional fungal identification depends on microscopic analysis and microorganism medium growth. Other diagnostic methods, non-growth dependent, to invasive fungal infections, are the biomarkers that detect circulating polysaccharides, for example, 1-3-β-d-Glucan and galactomannan. Both are polysaccharides present on the external layer of fungi cell wall and can be detected in clinical samples during the growth of the fungus in the patient. This study aimed to compare the galactomannan detection of Lateral Flow Assay and Enzyme Immunoassay methods in Bronchoalveolar Lavage Fluid. The galactomannan antigen in Bronchoalveolar Lavage Fluid was measured using Enzyme Immunoassay according to the manufacturer's instructions (PLATELIA ASPERGILLUS™ BioRad) and, using a Lateral Flow Assay according to the manufacturer's instructions (Galactomannan LFA IMMY©). The 71 samples were Bronchoalveolar Lavage Fluid of patients hospitalized at Unicamp Clinical Hospital between 2019 and 2021; of these samples 12/71 (16.9 %) resulted in positive Galactomannan-Lateral Flow Assay. In contrast, Galactomannan-Enzyme Immunoassay resulted as positive in 9/71 (12.6 %) samples, a difference that showed not significant statistically (p-value = 0.36) Comparing both assays' results identified 8 divergences between them, about 11 % of the total sample. The Sensitivity (73.3 %), Specificity (92.35 %), Positive Predictive Value (62.85 %) and Negative Predictive Value (95.15 %) of Lateral Flow Assay were calculated using the Galactomannan Enzyme Immunoassay as standard. The Lateral Flow Assay demonstrated good results when compared with the Enzyme Immunoassay. Competing Interests: Conflicts of interest The authors declare no have conflicts of interest. (Copyright © 2024 Sociedade Brasileira de Infectologia. Published by Elsevier España, S.L.U. All rights reserved.)
Databáze:	MEDLINE
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