CD66b + /CD68 + circulating extracellular vesicles, lactate dehydrogenase and neutrophil-to-lymphocyte ratio can differentiate coronavirus disease 2019 severity during and after infection.

Autor: Suades R; Cardiovascular Program ICCC, Institut de Recerca Sant Pau (IR SANT PAU), Barcelona, Spain.; Centro de Investigación Biomédica en Red Enfermedades Cardiovasculares (CIBERCV), Institute of Health Carlos III, Madrid, Spain., Greco MF; Cardiovascular Program ICCC, Institut de Recerca Sant Pau (IR SANT PAU), Barcelona, Spain., Prieto P; Infectious Diseases Unit, Department of Internal Medicine, Hospital de la Santa Creu i Sant Pau - IR SANT PAU, Barcelona, Spain., Padró T; Cardiovascular Program ICCC, Institut de Recerca Sant Pau (IR SANT PAU), Barcelona, Spain.; Centro de Investigación Biomédica en Red Enfermedades Cardiovasculares (CIBERCV), Institute of Health Carlos III, Madrid, Spain., Devaux Y; Cardiovascular Research Unit, Department of Precision Health, Luxembourg Institute of Health, Strassen, Luxembourg., Domingo P; Infectious Diseases Unit, Department of Internal Medicine, Hospital de la Santa Creu i Sant Pau - IR SANT PAU, Barcelona, Spain.; Universitat Autònoma de Barcelona (UAB), Barcelona, Spain., Badimon L; Cardiovascular Program ICCC, Institut de Recerca Sant Pau (IR SANT PAU), Barcelona, Spain.; Centro de Investigación Biomédica en Red Enfermedades Cardiovasculares (CIBERCV), Institute of Health Carlos III, Madrid, Spain.; Cardiovascular Research Chair, UAB, Barcelona, Spain.
Jazyk: angličtina
Zdroj: Journal of extracellular vesicles [J Extracell Vesicles] 2024 Jul; Vol. 13 (7), pp. e12456.
DOI: 10.1002/jev2.12456
Abstrakt: Coronavirus disease 2019 (COVID-19) has been a major public health burden. We hypothesised that circulating extracellular vesicles (cEVs), key players in health and disease, could trace the cell changes during COVID-19 infection and recovery. Therefore, we studied the temporal trend of cEV and inflammatory marker levels in plasma samples of COVID-19 patients that were collected within 24 h of patient admission (baseline, n = 80) and after hospital discharge at day-90 post-admission (n = 59). Inflammatory markers were measured by standard biochemical methods. cEVs were quantitatively and phenotypically characterized by high-sensitivity nano flow cytometry. In patients recovered from COVID-19 lower levels of inflammatory markers were detected. cEVs from vascular (endothelial cells) and blood (platelets, distinct immune subsets) cells were significantly reduced at day-90 compared to admission levels, a pattern also observed for cEVs from progenitor, perivascular and epithelial cells. The best discriminatory power for COVID-19 severity was found for inflammatory markers lactate dehydrogenase and neutrophil-to-lymphocyte ratio and for granulocyte/macrophage-released CD66b + /CD68 + -cEVs. Albeit inflammatory markers were good indicators of systemic inflammatory response and discriminators of COVID-19 remission, they do not completely reveal cell stress and organ damage states. cEVs reaching baseline pre-infection levels at 90 days post-infection in recovered patients discriminate parental cells affected by disease.
(© 2024 The Author(s). Journal of Extracellular Vesicles published by Wiley Periodicals LLC on behalf of International Society for Extracellular Vesicles.)
Databáze: MEDLINE
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