Potential nanomedicinal applications and physicochemical nature of Hyphaene thebaica-reduced nano-samaria.

Autor: Mohamed HEA; UNESCO UNISA Africa Chair in Nanoscience and Nanotechnology, College of Graduate Studies, University of South Africa, Pretoria, South Africa.; Nanoscience African Network (NANOAFNET), Materials Research Department, Cape Town, South Africa., Khalil AT; Department of Pathology, Lady Reading Hospital Medical Teaching Institution, Peshawar, Khyber Pakhtunkhwa, Pakistan., Hkiri K; UNESCO UNISA Africa Chair in Nanoscience and Nanotechnology, College of Graduate Studies, University of South Africa, Pretoria, South Africa.; Nanoscience African Network (NANOAFNET), Materials Research Department, Cape Town, South Africa., Ayaz M; Department of Pharmacy, Faculty of biological sciences, University of Malakand, Dir, Khyber Pakhtunkhwa, Pakistan., Usman A; Department of Pharmacy, Faculty of biological sciences, University of Malakand, Dir, Khyber Pakhtunkhwa, Pakistan., Sadiq A; Department of Pharmacy, Faculty of biological sciences, University of Malakand, Dir, Khyber Pakhtunkhwa, Pakistan., Ullah F; Department of Pharmacy, Faculty of biological sciences, University of Malakand, Dir, Khyber Pakhtunkhwa, Pakistan., Khan MA; Department of Pharmacy, Faculty of biological sciences, University of Malakand, Dir, Khyber Pakhtunkhwa, Pakistan., Ullah I; Department of Biotechnology and Genetic Engineering, Hazara University, Mansehra, Khyber Pakhtunkhwa, Pakistan., Maaza M; UNESCO UNISA Africa Chair in Nanoscience and Nanotechnology, College of Graduate Studies, University of South Africa, Pretoria, South Africa.; Nanoscience African Network (NANOAFNET), Materials Research Department, Cape Town, South Africa.
Jazyk: angličtina
Zdroj: Microscopy research and technique [Microsc Res Tech] 2024 Dec; Vol. 87 (12), pp. 2829-2841. Date of Electronic Publication: 2024 Jul 15.
DOI: 10.1002/jemt.24654
Abstrakt: Herein we described the biofabrication of samarium oxide nanoparticles (HT-Sm 2 O 3 NPs) by applying the aqueous fruit extract of Hyphaene thebaica was utilized as an eco-friendly chelating agent. The prepared NPs were subjected to various physicochemical properties and potential in biomedical applications. X-ray Diffraction (XRD) pattern revealed sharp peaks that corroborated with the Joint Committee on Powder Diffraction Standards (JCPDS) card no. 00-042-1464. Crystallite size obtained from Debye-Scherrer approximation and Williamson-Hall (W-H) plot was 28.73 and 69.3 nm, respectively. Optical bandgap was calculated by employing Kubelka-Munk (K-M) function and was found to be ~4.58 eV. Raman shift was observed at 121, 351, 424 - , and 561 cm -1 . Photoluminescence (PL) spectra revealed two major peaks positioned at 360 and 540 nm. The high-resolution transmission electron microscopy (HR-TEM) analysis of HT-Sm 2 O 3 nanoparticles (NPs) showed that they predominantly have spherical to cuboidal shapes. Additionally, the selected area electron diffraction (SAED) pattern presented spotty rings, indicating a high level of crystallinity in these NPs. The potential nanomedicine applications were studied using diverse bioassays using different treatments. The antioxidant activity demonstrated 45.71% ± 1.13% inhibition at 1000 μg/mL. Brine shrimp lethality assay revealed the highest cytotoxicity of 46.67% ± 3.33% at 1000 μg/mL and LC 50 value of 1081 μg/mL. HT-Sm 2 O 3 NPs exhibited inhibition of angiogenesis (20.41% ± 1.18%) at of 1000 μg/mL. MTT assay results indicated that HT-Sm 2 O 3 NPs exhibit inhibitory effects on cell lines. Specifically, these NPs showed an IC 50 value of 104.6 μg/mL against 3T3 cells. Against MCF-7 cells, the NPs demonstrated an IC 50 value of 413.25 μg/mL. Additionally, in the inhibition of acetylcholinesterase (AChE), the newly synthesized NPs showed an IC 50 value of 320 μg/mL. The antidiabetic assessment through α-glucosidase and α-amylase inhibition assays revealed, an IC 50 value of 380 μg/mL for α-glucosidase and 952 μg/mL for α-amylase was calculated. Overall, our study suggested that the Sm 2 O 3 NPs possess moderate anticancer, cholinesterase inhibition, and antidiabetic potential, however, needs further assessment. RESEARCH HIGHLIGHTS: In this work, nano-samaria is synthesized using an eco-friendly and green approach. The nanoparticles were characterized using techniques such as Raman, HR-TEM, FTIR, DRS, XRD, and so on, and the applications were studied using multiple in vitro bioassays for Diabetes, Alzheimer, and Cancer. The nano-samaria revealed good potential for potential biomedical applications.
(© 2024 Wiley Periodicals LLC.)
Databáze: MEDLINE
Externí odkaz: