MENSA, a Media Enriched with Newly Synthesized Antibodies, to Identify SARS-CoV-2 Persistence and Latent Viral Reactivation in Long-COVID.

Autor:	Haddad NS; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; MicroB-plex Inc, Atlanta, GA, 30332, USA., Morrison-Porter A; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; MicroB-plex Inc, Atlanta, GA, 30332, USA., Quehl H; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Capric V; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Lamothe PA; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Anam F; Division of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Runnstrom MC; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Department of Medicine, Atlanta Veterans Affairs Health Care System, Decatur, Georgia, 30033, USA., Truong AD; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Dixit AN; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Woodruff MC; Division of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Lowance Center for Human Immunology, Emory University, Atlanta, GA, 30322, USA., Chen A; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Park J; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Nguyen DC; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Hentenaar I; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Kim CY; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Kyu S; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, 30322, USA., Stewart B; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Wagman E; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Geoffroy H; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Sanz D; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; MicroB-plex Inc, Atlanta, GA, 30332, USA.; Division of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Department of Medicine, Atlanta Veterans Affairs Health Care System, Decatur, Georgia, 30033, USA.; Lowance Center for Human Immunology, Emory University, Atlanta, GA, 30322, USA.; Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, 30322, USA.; Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, 15213, USA.; Asthma and Environmental Lung Health Institute, University of Pittsburgh Medical Center, Pittsburgh, PA, 15213, USA.; Division of General Internal Medicine, Department of Medicine, Emory University School of Medicine, Atlanta, GA, 30322, USA.; Metro Infectious Disease Consultants, Decatur GA 30033, USA.; ImmuneID, Inc Biotechnology Research, Waltham, MA, 02451, USA., Cashman KS; Division of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Lowance Center for Human Immunology, Emory University, Atlanta, GA, 30322, USA., Ramonell RP; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Division of Pulmonary, Allergy, Critical Care, and Sleep Medicine, Department of Medicine, University of Pittsburgh, Pittsburgh, PA, 15213, USA.; Asthma and Environmental Lung Health Institute, University of Pittsburgh Medical Center, Pittsburgh, PA, 15213, USA., Cabrera-Mora M; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Alter DN; Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, 30322, USA., Roback JD; Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, 30322, USA., Horwath MC; Department of Pathology and Laboratory Medicine, Emory University, Atlanta, GA, 30322, USA., O'Keefe JB; Division of General Internal Medicine, Department of Medicine, Emory University School of Medicine, Atlanta, GA, 30322, USA., Dretler AW; Metro Infectious Disease Consultants, Decatur GA 30033, USA., Gripaldo R; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Yeligar SM; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Department of Medicine, Atlanta Veterans Affairs Health Care System, Decatur, Georgia, 30033, USA., Natoli T; ImmuneID, Inc Biotechnology Research, Waltham, MA, 02451, USA., Betin V; ImmuneID, Inc Biotechnology Research, Waltham, MA, 02451, USA., Patel R; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Division of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Vela K; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Division of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Hernandez MR; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Division of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Usman S; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Division of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Varghese J; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Division of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Jalal A; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Division of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Lee S; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Division of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Le SN; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Amoss RT; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Division of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA., Daiss JL; MicroB-plex Inc, Atlanta, GA, 30332, USA., Sanz I; Division of Rheumatology, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Lowance Center for Human Immunology, Emory University, Atlanta, GA, 30322, USA., Lee FE; Division of Pulmonary, Allergy, Critical Care & Sleep Medicine, Department of Medicine, Emory University, Atlanta, GA, 30322, USA.; Lowance Center for Human Immunology, Emory University, Atlanta, GA, 30322, USA.
Jazyk:	angličtina
Zdroj:	MedRxiv : the preprint server for health sciences [medRxiv] 2024 Jul 07. Date of Electronic Publication: 2024 Jul 07.
DOI:	10.1101/2024.07.05.24310017
Abstrakt:	Post-acute sequelae of SARS-CoV-2 (SARS2) infection (PASC) is a heterogeneous condition, but the main viral drivers are unknown. Here, we use MENSA, Media Enriched with Newly Synthesized Antibodies, secreted exclusively from circulating human plasmablasts, to provide an immune snapshot that defines the underlying viral triggers. We provide proof-of-concept testing that the MENSA technology can capture the new host immune response to accurately diagnose acute primary and breakthrough infections when known SARS2 virus or proteins are present. It is also positive after vaccination when spike proteins elicit an acute immune response. Applying the same principles for long-COVID patients, MENSA is positive for SARS2 in 40% of PASC vs none of the COVID recovered (CR) patients without any sequelae demonstrating ongoing SARS2 viral inflammation only in PASC. Additionally, in PASC patients, MENSAs are also positive for Epstein-Barr Virus (EBV) in 37%, Human Cytomegalovirus (CMV) in 23%, and herpes simplex virus 2 (HSV2) in 15% compared to 17%, 4%, and 4% in CR controls respectively. Combined, a total of 60% of PASC patients have a positive MENSA for SARS2, EBV, CMV, and/or HSV2. MENSA offers a unique antibody snapshot to reveal the underlying viral drivers in long-COVID thus demonstrating the persistence of SARS2 and reactivation of viral herpes in 60% of PASC patients. Competing Interests: Declaration of Potential Conflicts of Interest FEL is the founder of MicroB-plex, Inc. and serves on the scientific board of Be Biopharma, is a recipient of grants from the BMGF and Genentech, Inc., and has served as a consultant for Astra Zeneca. NSH and AMP were scientists at MicroB-plex, Inc., Atlanta, GA and JLD is a scientist at MicroB-plex, Inc., Atlanta, GA. IS has consulted for GSK, Pfizer, Kayverna, Johnson & Johnson, Celgene, Bristol Myer Squibb, and Visterra. FEL, DN, and IS are inventors of the patents concerning the plasma cell survival media related to this work (issued 9/21/21, US 11,124766 B2 PCT/US2016/036650; and issued 9/21/21, US 11,125757 B2). FEL & JLD are inventors of MENSA patent U.S. Patent No. 10,247,729. April 2, 2019. FEL, NSH, JLD, & IS are inventors of the MENSA PASC diagnostic provisional patent, March 28, 2024. All other authors have declared that no conflict of interest exists.
Databáze:	MEDLINE
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