Immunogenicity of mannan derived from Mycobacterium bovis as a promising adjuvant in vaccine BCG.

Autor: Mardani R; Department of Viral Vaccines, Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran., Alavi A; Department of Production, Bsc in Biotechnology Pharmaceutical and Drug Delivery System Labratoary, Pasteur Institute of Iran, Tehren, Iran., Mousavi Nasab SD; Department of Arboviruses and Viral Hemorrhagic Fevers (National Ref Lab), Pasteur Institute of Iran, Tehran, Iran., Ahmadi N; Proteomics Research Center, Department of Medical Lab Technology, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran., Hossein Tehrani MJ; Department of Clinical Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran., Shahali M; Department of Viral Vaccines, Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran., Doroud D; Department of Production, Research and Production Complex, Pasteur Institute of Iran, Tehran, Iran.
Jazyk: angličtina
Zdroj: Iranian journal of microbiology [Iran J Microbiol] 2024 Jun; Vol. 16 (3), pp. 351-356.
DOI: 10.18502/ijm.v16i3.15767
Abstrakt: Background and Objectives: Lipoarabinomannan is one of the components of the significant structural cell surfaces of mycobacteria and serves as an immunostimulatory factor. TNF-α and IL-12 are two examples of the anti-bacterial inflammatory cytokines that are activated and induced during infection.
Materials and Methods: In this study, mannan was extracted and processed, and then Bulb/c female mice were used in three groups, one group was given BCG vaccine, the other group was given BCG vaccine with mannan adjuvant, and a non-injected group was used as a control group. Inflammatory factors interleukin-12, TNF-α, IgG and IgM were measured in mouse serum.
Results: The levels of the inflammatory factors interleukin-12 and TNF-α in the serum isolated from mice receiving the BCG vaccine with mannan adjuvant showed a significant difference compared to the group that received only the BCG vaccine and the control group [IL-12] and , with P≤0.05.The examination of the level of IgG immune factors in these three groups revealed a significant difference. The group that received the BCG vaccine with mannan adjuvant showed a marked contrast compared to the group that received only the BCG vaccine and the control group, with P≤0.05. The level of IgM was higher in the group that received the BCG vaccine alone compared to the adjuvant vaccine group and the control group, with P≤0.05.
Conclusion: Our results indicated that mice receiving the BCG vaccine with mannan adjuvant had significantly higher serum levels of IL-12, TNF-α, and IgG than the group receiving BCG alone.
(Copyright© 2024 The Authors. Published by Tehran University of Medical Sciences.)
Databáze: MEDLINE
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