Con-DAM: Simultaneous measurement of food intake and sleep in Drosophila at the single fly resolution.

Autor:	Beard B; Department of Biology, University of Louisville, Louisville, Kentucky, United States., Bohn A; Department of Biology, University of Louisville, Louisville, Kentucky, United States., Opoola M; Department of Biology, University of Louisville, Louisville, Kentucky, United States., Hwangbo DS; Department of Biology, University of Louisville, Louisville, Kentucky, United States.
Jazyk:	angličtina
Zdroj:	MicroPublication biology [MicroPubl Biol] 2024 Jun 30; Vol. 2024. Date of Electronic Publication: 2024 Jun 30 (Print Publication: 2024).
DOI:	10.17912/micropub.biology.001200
Abstrakt:	Sleep and feeding are conserved behaviors across many taxa of the animal kingdom and are essential for an organism's survival and fitness. Although Drosophila has been used to study these behaviors for decades, concurrent measurement of these two behaviors in the same flies on solid media has been a challenge. Here, we report Con-DAM, which enables simultaneous quantification of food intake and sleep/activity at the single fly resolution. Since Con-DAM integrates the Con-Ex (Consumption-Excretion) assay and the DAM (Drosophila Activity Monitor), two widely used tools to quantify food consumption and sleep/activity in flies into a single unit, we expect Con-DAM to serve as an easy method for various purposes that require quantifying food consumption and sleep/activity in the same individual flies. Competing Interests: The authors declare that there are no conflicts of interest present. (Copyright: © 2024 by the authors.)
Databáze:	MEDLINE
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