FlhE functions as a chaperone to prevent formation of periplasmic flagella in Gram-negative bacteria.

Autor: Halte M; Institute of Biology, Humboldt-Universität zu Berlin, Philippstr. 13, 10115, Berlin, Germany. manuel.halte@hu-berlin.de., Andrianova EP; Department of Microbiology, The Ohio State University, Columbus, OH, 43210, USA., Goosmann C; Max Planck Institute for Infection Biology, Charitéplatz 1, 10117, Berlin, Germany., Chevance FFV; School of Biological Sciences, University of Utah, Salt Lake City, UT, USA., Hughes KT; School of Biological Sciences, University of Utah, Salt Lake City, UT, USA., Zhulin IB; Department of Microbiology, The Ohio State University, Columbus, OH, 43210, USA., Erhardt M; Institute of Biology, Humboldt-Universität zu Berlin, Philippstr. 13, 10115, Berlin, Germany. marc.erhardt@hu-berlin.de.; Max Planck Unit for the Science of Pathogens, Charitéplatz 1, 10117, Berlin, Germany. marc.erhardt@hu-berlin.de.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2024 Jul 14; Vol. 15 (1), pp. 5921. Date of Electronic Publication: 2024 Jul 14.
DOI: 10.1038/s41467-024-50278-0
Abstrakt: The bacterial flagellum, which facilitates motility, is composed of ~20 structural proteins organized into a long extracellular filament connected to a cytoplasmic rotor-stator complex via a periplasmic rod. Flagellum assembly is regulated by multiple checkpoints that ensure an ordered gene expression pattern coupled to the assembly of the various building blocks. Here, we use epifluorescence, super-resolution, and transmission electron microscopy to show that the absence of a periplasmic protein (FlhE) prevents proper flagellar morphogenesis and results in the formation of periplasmic flagella in Salmonella enterica. The periplasmic flagella disrupt cell wall synthesis, leading to a loss of normal cell morphology resulting in cell lysis. We propose that FlhE functions as a periplasmic chaperone to control assembly of the periplasmic rod, thus preventing formation of periplasmic flagella.
(© 2024. The Author(s).)
Databáze: MEDLINE
Externí odkaz: