Antifungal Activity of Piperine-based Nanoemulsion Against Candida spp. via In Vitro Broth Microdilution Assay.

Autor:	Adisuri DS; School of Medicine, Taylor's University Lakeside Campus, Subang Jaya 47500, Malaysia; Digital Health and Medical Advancement Impact Lab, Taylor's University, No.1, Jalan Taylor's. Subang Jaya, 47500 Selangor, Malaysia., Madhavan P; School of Medicine, Taylor's University Lakeside Campus, Subang Jaya 47500, Malaysia; Digital Health and Medical Advancement Impact Lab, Taylor's University, No.1, Jalan Taylor's. Subang Jaya, 47500 Selangor, Malaysia. Electronic address: Priya.Madhavan@taylors.edu.my., Chong PP; School of Biosciences, Taylor's University Lakeside Campus, 47500 Subang Jaya, Malaysia; Digital Health and Medical Advancement Impact Lab, Taylor's University, No.1, Jalan Taylor's. Subang Jaya, 47500 Selangor, Malaysia., Nathan S; School of Biosciences and Biotechnology, Universiti Kebangsaan Malaysia, Bangi 43600, Malaysia.
Jazyk:	angličtina
Zdroj:	Journal of pharmaceutical sciences [J Pharm Sci] 2024 Sep; Vol. 113 (9), pp. 2843-2850. Date of Electronic Publication: 2024 Jul 14.
DOI:	10.1016/j.xphs.2024.06.024
Abstrakt:	Candidemia leaves a trail of approximately 750,000 cases yearly, with a morbidity rate of up to 30%. While Candida albicans still ranks as the most predominantly isolated Candida species, C. glabrata comes in second, with a death rate of 40-50%. Although infections by Candida spp are commonly treated with azoles, the side effects and rise in resistance against it has significantly limited its clinical usage. The current study aims to address the insolubility of piperine and provide an alternative treatment to Candida infection by formulating a stable piperine-loaded O/W nanoemulsion, comprised of Cremophor RH40, Transcutol HP and Capryol 90 as surfactant, co-surfactant, and oil, respectively. Characterization with zetasizer showed the droplet size, polydispersity (PDI) and zetapotential value of the nanoemulsion to be 24.37 nm, 0.453 and -21.10 mV, respectively, with no observable physical changes such as phase separation from thermostability tests. FTIR peaks confirms presence of piperine within the nanoemulsion and TEM imaging visualized the droplet shape and further confirms the droplet size range of 20-24 nm. The MIC90 value of the piperine-loaded nanoemulsion determined with in vitro broth microdilution assay was approximately 20-50% lower than that of the pure piperine in DMSO, at a range of 0.8-2.0 mg/mL across all Candida spp. tested. Overall, the study showed that piperine can be formulated into a stable nanoemulsion, which significantly enhances its antifungal activity compared to piperine in DMSO. Competing Interests: Declaration of competing interest All the authors ensure that there is no conflict of interest regarding authorship or any other matters pertaining to this manuscript. (Copyright © 2024 American Pharmacists Association. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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