Mathematical modelling of genipin-bovine serum albumin interaction using fluorescence intensity measurements.

Autor: Vukajlovic D; School of Engineering, Newcastle University, Newcastle upon Tyne NE1 7RU, United Kingdom., Timmons R; School of Engineering, Newcastle University, Newcastle upon Tyne NE1 7RU, United Kingdom., Macesic S; University of Belgrade, Faculty of Physical Chemistry, Studentski trg 12-16, Belgrade, Serbia., Sanderson J; School of Engineering, Newcastle University, Newcastle upon Tyne NE1 7RU, United Kingdom., Xie F; School of Engineering, Newcastle University, Newcastle upon Tyne NE1 7RU, United Kingdom; Department of Chemical Engineering, University of Bath, Bath BA2 7AY, United Kingdom., Abdelghany TM; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., Cairo 11562, Egypt; Institute of Education in Healthcare and Medical Sciences, School of Medicine, Medical Sciences and Nutrition, University of Aberdeen, Foresthill, Aberdeen AB25 2ZD, United Kingdom; School of Biomedical, Nutritional and Sport Sciences, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE2 4HH, United Kingdom., Smith E; School of Pharmacy, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, United Kingdom., Lau WM; School of Pharmacy, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, United Kingdom., Ng KW; School of Pharmacy, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, United Kingdom., Novakovic K; School of Engineering, Newcastle University, Newcastle upon Tyne NE1 7RU, United Kingdom. Electronic address: katarina.novakovic@newcastle.ac.uk.
Jazyk: angličtina
Zdroj: International journal of biological macromolecules [Int J Biol Macromol] 2024 Sep; Vol. 276 (Pt 1), pp. 133850. Date of Electronic Publication: 2024 Jul 14.
DOI: 10.1016/j.ijbiomac.2024.133850
Abstrakt: The interaction between genipin and a model protein bovine serum albumin (BSA), with and without the addition of acetic acid, has been studied experimentally and by modelling. The number of amino groups available to react was determined to be 5.6 % of the total number of amino acid building blocks on BSA. Fluorescence intensity was used to record the progress of the reaction over the 24 h, while the modelling study focused on capturing the kinetic profiles of the reaction. The experiments revealed a slow start to the BSA and genipin interaction, that subsequently accelerated in an S-shaped curve which the modelling study linked with the existence of the feedback cycle for both reactive amino groups and genipin. At BSA concentrations ≥30 mg/mL the reaction was accelerated in the presence of acid, while below 30 mg/mL the acidified conditions delayed the onset of the reaction. Contrary to the reaction mechanisms previously proposed, a degree of breakdown of the fluorescent links in the products formed was denoted both experimentally and in a modelling study. This indicated the reversibility of the processes forming fluorescent product/s and suggested feasibility of the successful release of the protein following prospective encapsulation within the genipin-crosslinked hydrogel structure.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Katarina Novakovic reports financial support was provided by The Northern Accelerator. Stevan Macesic reports financial support was provided by Ministry of Science, Technological Development and Innovation of the Republic of Serbia. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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