Development of an entirely cloned cDNA-based reverse genetics system for Tofla virus of orthonairovirus.

Autor: Wulandari S; Laboratory of Veterinary Microbiology, Joint Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi, 753-8511, Japan; Department of Health, Faculty of Vocational Studies, Universitas Airlangga, Surabaya, 60286, Indonesia., Nyampong S; Laboratory of Veterinary Microbiology, Joint Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi, 753-8511, Japan., Lokupathirage SMW; Institute for Genetic Medicine, Hokkaido University, Sapporo, 060-0815, Japan., Yoshimatsu K; Institute for Genetic Medicine, Hokkaido University, Sapporo, 060-0815, Japan., Shimoda H; Laboratory of Veterinary Microbiology, Joint Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi, 753-8511, Japan; Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, 753-8511, Japan., Hayasaka D; Laboratory of Veterinary Microbiology, Joint Graduate School of Veterinary Medicine, Yamaguchi University, Yamaguchi, 753-8511, Japan; Laboratory of Veterinary Microbiology, Joint Faculty of Veterinary Medicine, Yamaguchi University, Yamaguchi, 753-8511, Japan. Electronic address: dhaya@yamaguchi-u.ac.jp.
Jazyk: angličtina
Zdroj: Virology [Virology] 2024 Oct; Vol. 598, pp. 110170. Date of Electronic Publication: 2024 Jul 09.
DOI: 10.1016/j.virol.2024.110170
Abstrakt: The genus Orthonairovirus includes highly pathogenic tick-borne viruses such as the Crimean-Congo hemorrhagic fever orthonairovirus (CCHFV). A reverse genetics system is an indispensable tool for determining the viral factors related to pathogenicity. Tofla orthonairovirus (TFLV) is a recently identified virus isolated from ticks in Japan and our research has suggested that TFLV is a useful model for studying pathogenic orthonairoviruses. In this study, we successfully established a reverse genetics system for TFLV using T7 RNA polymerase. Recombinant TFLV was generated by transfecting cloned complementary DNAs encoding the TFLV genome into BSR T7/5 cells expressing T7 RNA polymerase. We were able to rescue infectious recombinant TFLV mutant (rTFLVmt) and wild-type TFLV (rTFLVpt) viruses, which exhibited indistinguishable growth kinetics in mammalian cells and pathogenicity in A129 mice compared with the authentic virus. Our approach provides a valuable method for establishing reverse genetics system for orthonairoviruses.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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