Superparamagnetic photonic crystals with DNA probes for rapid visual detection of mercury.

Autor: Pu Y; Yunnan Normal University, College of Vocational Education, Kunming 650092, China., Gu Z; Kunming Institute of Botany, Chinese Academy of Sciences, Key Laboratory for Plant Biodiversity and Biogeography of East Asia, Kunming 650201, China., Bovee TFH; Wageningen University & Research, Wageningen Food Safety Research (WFSR), Wageningen, 6708WB, Netherlands., Yang Y; Yunnan Normal University, College of Vocational Education, Kunming 650092, China., Ying Y; Yunnan Normal University, College of Vocational Education, Kunming 650092, China., Li M; Yunnan Normal University, College of Vocational Education, Kunming 650092, China., Hong X; Yunnan Normal University, College of Vocational Education, Kunming 650092, China; Max Planck Institute for Medical Research, Department of Cellular Biophysics, Heidelberg, 69120, Germany. Electronic address: xiaodihong@hotmail.com.
Jazyk: angličtina
Zdroj: Food chemistry [Food Chem] 2024 Nov 30; Vol. 459, pp. 140354. Date of Electronic Publication: 2024 Jul 04.
DOI: 10.1016/j.foodchem.2024.140354
Abstrakt: A novel superparamagnetic photonic crystal DNA probe (Fe 3 O 4 @SiO 2 @amino@DNA SPC) was developed to enable rapid visual detection of Hg 2+ . This unique photonic crystal (PC) was synthesized by combining superparamagnetic nanospheres with DNA probes. The DNA probe, rich in thymine (T), detects mercury ions through base mismatch, resulting in the formation of T-Hg 2+ -T loop hairpin structures. With the binding of Hg 2+ to the probe attached to superparamagnetic nanospheres, the PC structure assembled by these nanospheres, formed by the magnetic field, was changed. This change enhanced the reflection intensity; it could be quantified using a fiber optic spectrometer and was visible to the naked eye. The Fe 3 O 4 @SiO 2 @amino@DNA SPC, specific to Hg 2+ , exhibited a reflection peak at 679 nm, which intensified with increasing Hg 2+ concentration. The reflection intensity increased by 132.58 a.u., and the PC color shifted from red to yellow as the Hg 2+ concentration increased from 0.1 μg/L to 1 mg/L.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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