EROD activity in the native fish Cnesterodon decemmaculatus as a biomarker for assessing aquatic pollution by AhR agonist chemicals within the Rio de la Plata Basin.

Autor: Rabuffetti G; Centro de Investigaciones del Medioambiente (CIM), UNLP - CONICET, Boulevard 120 N1489, 1900, La Plata, Buenos Aires, Argentina., Brola J; Centro de Investigaciones del Medioambiente (CIM), UNLP - CONICET, Boulevard 120 N1489, 1900, La Plata, Buenos Aires, Argentina., Pérez D; Centro de Investigaciones del Medioambiente (CIM), UNLP - CONICET, Boulevard 120 N1489, 1900, La Plata, Buenos Aires, Argentina., Somoza GM; Instituto Tecnológico de Chascomús (CONICET-UNSAM), Av. Intendente Marino Km. 8,2, B7130IWA, Chascomús, Provincia de Buenos Aires, Argentina; Escuela de Bio y Nanotecnologías (UNSAM), Argentina., Carriquiriborde P; Centro de Investigaciones del Medioambiente (CIM), UNLP - CONICET, Boulevard 120 N1489, 1900, La Plata, Buenos Aires, Argentina. Electronic address: pcarriquiriborde@gmail.com.
Jazyk: angličtina
Zdroj: The Science of the total environment [Sci Total Environ] 2024 Oct 15; Vol. 947, pp. 174721. Date of Electronic Publication: 2024 Jul 11.
DOI: 10.1016/j.scitotenv.2024.174721
Abstrakt: The 7-ethoxyresorufin-O-deethylase (EROD) activity was first time characterized in the neotropical fish Cnesterodon decemmaculatus as a biomarker for assessing environmental health in aquatic ecosystems of the Rio de la Plata Basin impacted by organic pollutants agonist of the aryl-hydrocarbon receptor (AhR). Both laboratory and field studies were conducted. Laboratory experiments were run using β-naphthoflavone (BNF) as an AhR agonist model. A clear concentration-response relationship was found between 1 and 100 μg/L, with a NOEC and LOEC of 1 and 10 μg/L. A fast time-dependent response was observed with a significant induction after 24 h and a plateau from 24 to 48 h up to 264 h of exposure. Differences in basal activity were found between juveniles, females, and males, but induction levels were similar. Both basal activities and induction levels were distinct in the whole body, liver, gill, muscle, brain, and embryos. Fold-change inductions in the respective tissues were: 20, 114, 3, 5, 1, and 14. Maternal transfer and early cyp1a activation were unveiled by embryonic induction. Clear differences in EROD activity were found among juveniles collected in hydrocarbon-polluted streams, beside the La Plata Petrochemical hub, and a reference stream. Similar EROD activities were observed in laboratory and feral fish, usually with values below or above 1,000 pmol/min x mg protein for unexposed or exposed organisms. The study contributes with original information about EROD activity in C. decemmaculatus that encourages the use of both the response as a robust biomarker of exposure and the species as a good sentinel organism to be included in surveillant programs for assessing aquatic pollution by AhR agonist chemicals within the Rio de la Plata Basin within the One Health paradigm.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier B.V.)
Databáze: MEDLINE
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