Urinary neutrophil gelatinase-associated lipocalin as early biomarker for renal disease in dogs with leishmaniosis.

Autor: Ruiz P; MINVET Research Group. Departamento de Medicina Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres 10003, Spain., Durán Á; Hospital Clínico Veterinario, Facultad de Veterinaria, Universidad de Extremadura, Cáceres 10003, Spain., Gil M; Hospital Clínico Veterinario, Facultad de Veterinaria, Universidad de Extremadura, Cáceres 10003, Spain., Sevidane I; Hospital Clínico Veterinario, Facultad de Veterinaria, Universidad de Extremadura, Cáceres 10003, Spain., Cristóbal JI; MINVET Research Group. Departamento de Medicina Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres 10003, Spain., Nicolás P; MINVET Research Group. Departamento de Medicina Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres 10003, Spain., Duque FJ; MINVET Research Group. Departamento de Medicina Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres 10003, Spain., Zaragoza C; MINVET Research Group. Departamento de Medicina Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres 10003, Spain., García AB; Hospital Clínico Veterinario, Facultad de Veterinaria, Universidad de Extremadura, Cáceres 10003, Spain., Macías-García B; MINVET Research Group. Departamento de Medicina Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres 10003, Spain. Electronic address: bemaciasg@unex.es., Barrera R; MINVET Research Group. Departamento de Medicina Animal, Facultad de Veterinaria, Universidad de Extremadura, Cáceres 10003, Spain.
Jazyk: angličtina
Zdroj: Veterinary parasitology [Vet Parasitol] 2024 Oct; Vol. 331, pp. 110251. Date of Electronic Publication: 2024 Jul 10.
DOI: 10.1016/j.vetpar.2024.110251
Abstrakt: Canine leishmaniosis (CanL), caused by Leishmania sp., presents a wide array of symptoms; renal dysfunction is frequently observed in these dogs and is associated with a poor prognosis and increased mortality. The traditional biomarkers namely urea and creatinine can detect renal damage but only in advanced stages of the disease. However, it has been shown that the symmetric dimethylarginine assay (SDMA) or the protein/creatinine ratio (UPC) and are early biomarkers of renal dysfunction. Their elevation occurs earlier than that of creatinine, but other novel biomarkers such as neutrophil gelatinase-associated lipocalin (NGAL) are currently under investigation. Our objective was to determine whether the urine NGAL-creatinine ratio (uNGAL/c) can provide very early diagnosis of kidney disease in CanL. In total, 68 dogs were included in the study: 15 healthy dogs and 53 dogs with CanL who were classified according to International Renal Interest Society (IRIS) classification: IRIS 1 (N= 34), IRIS 2 (N= 9) and IRIS 3/4 (N= 10). IRIS 1 was subdivided according to proteinuria in IRIS 1 NP (13 dogs with UPC < 0.2), IRIS 1 BL (8 dogs with UPC = 0.2-0.5) and IRIS 1 P (13 dogs with UPC > 0.5). Blood samples were collected for complete hematological and biochemistry analysis including plasma NGAL. Urinalysis included specific gravity, UPC, CysC and NGAL expressed as a ratio with creatinine. The mean concentrations of pCysC and SDMA in CanL, show a statistically significant increase from IRIS 1 NP , not being statistically significant for pCysC in the IRIS 1 BL group. The UPC show a statistically significant increase from IRIS 1 NP. In all groups with CanL for uCysC/c and uNGAL/c was observed a statistically significant increase. The uNGAL/c in the group proteinuric animals, presents a positive correlation with all renal biomarkers studied. In the group of non-proteinuric animals, the uNGAL/c presents a positive correlation with SDMA and UPC. The uNGAL/c can be considered a reliable indicator of renal disease in dogs diagnosed with CanL who are non-azotemic and non-proteinuric.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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