Proinflammatory Activation of Monocytes in Patients with Immunoinflammatory Rheumatic Diseases.

Autor: Bogatyreva AI; Avtsyn Research Institute of Human Morphology, Petrovsky Russian Scientific Center of Surgery, Moscow, Russia.; Nasonova Research Institute of Rheumatology, Moscow, Russia., Gerasimova EV; Nasonova Research Institute of Rheumatology, Moscow, Russia. gerasimovaev@list.ru., Kirichenko TV; Avtsyn Research Institute of Human Morphology, Petrovsky Russian Scientific Center of Surgery, Moscow, Russia., Markina YV; Avtsyn Research Institute of Human Morphology, Petrovsky Russian Scientific Center of Surgery, Moscow, Russia., Popkova TV; Nasonova Research Institute of Rheumatology, Moscow, Russia., Shalygina MV; Nasonova Research Institute of Rheumatology, Moscow, Russia., Tolstik TV; Avtsyn Research Institute of Human Morphology, Petrovsky Russian Scientific Center of Surgery, Moscow, Russia., Markin AM; Avtsyn Research Institute of Human Morphology, Petrovsky Russian Scientific Center of Surgery, Moscow, Russia., Orekhov AN; Avtsyn Research Institute of Human Morphology, Petrovsky Russian Scientific Center of Surgery, Moscow, Russia.
Jazyk: angličtina
Zdroj: Doklady. Biochemistry and biophysics [Dokl Biochem Biophys] 2024 Aug; Vol. 517 (1), pp. 228-234. Date of Electronic Publication: 2024 Jul 13.
DOI: 10.1134/S1607672924700959
Abstrakt: The pathogenesis of immunoinflammatory rheumatic diseases (IRDs) is based on chronic inflammation, one of the key mechanisms of which may be abnormal activation of macrophages, leading to further disruption of the immune system.
Objective: . The objective of this study was to evaluate the proinflammatory activation of circulating monocytes in patients with IRDs.
Materials and Methods: . The study involved 149 participants (53 patients with rheumatoid arthritis (RA), 45 patients with systemic lupus erythematosus (SLE), 34 patients with systemic scleroderma (SSc), and 17 participants without IRDs) 30 to 65 years old. Basal and lipopolysaccharide (LPS)-stimulated secretion of monocytes was studied in a primary culture of monocytes obtained from blood by immunomagnetic separation. Quantitative assessment of the cytokines tumor necrosis factor α (TNF-α), interleukin 1β (IL-1β), as well as the chemokine monocyte chemoattractant protein-1 (MCP-1) was carried out in the culture fluid by ELISA. Proinflammatory activation of monocytes was calculated as the ratio of LPS-stimulated and basal secretions.
Results: . It was shown that the basal secretion of all studied cytokines was significantly increased in all groups of patients with IRDs, except for the secretion of IL-1β in the SLE group, compared to the control. LPS-stimulated secretion of TNF-α was increased and MCP-1 was decreased in patients with IRDs compared to the control group; LPS-stimulated IL-1β secretion only in the SSc group significantly differed from the control group. In the RA group, monocyte activation was reduced for all cytokines compared to the control; in the SLE group, for TNF-α and MCP-1; in the SSc group, for MCP-1.
Conclusions: . The decrease in proinflammatory activation of monocytes in patients with IRDs is due to a high level of basal secretion of cytokines, which can lead to disruption of the adequate immune response in these diseases and is an important link in the pathogenesis of chronic inflammation.
(© 2024. Pleiades Publishing, Ltd.)
Databáze: MEDLINE
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