| Autor: |
Fernández Míguez M; Department of Biological Sciences, University of Bergen, 5006 Bergen, Norway.; Laboratory of Marine Genetic Resources, ReXenMar, CIM, Universidade de Vigo, 36310 Vigo, Spain.; AQUACOV, Centro Oceanográfico de Vigo, Instituto Español de Oceanografía (IEO, CSIC), 36202 Vigo, Spain., Presa P; Laboratory of Marine Genetic Resources, ReXenMar, CIM, Universidade de Vigo, 36310 Vigo, Spain., Puvanendran V; Department of Production Biology, Centre for Marine Aquaculture, Nofima AS, 9291 Tromsø, Norway., Tveiten H; Faculty of Biosciences, Fisheries and Economics, Norwegian College of Fishery Science, The Arctic University of Norway (UiT), 9019 Tromsø, Norway., Hansen ØJ; Department of Production Biology, Centre for Marine Aquaculture, Nofima AS, 9291 Tromsø, Norway., Pérez M; AQUACOV, Centro Oceanográfico de Vigo, Instituto Español de Oceanografía (IEO, CSIC), 36202 Vigo, Spain. |
| Abstrakt: |
Egg quality in fishes is commonly determined by fertilisation success and cleavage patterns as a phenotypic outcome of underlying regulatory mechanisms. Although these phenotypic estimators of egg quality are useful in farming conditions, these "good quality" egg batches do not always translate to good larval growth and survival. The identification of genes involved in embryonic development may help find links between genetic factors of maternal origin and egg quality. Herein, the relative expression of seven stage-specific developmental genes of Atlantic cod was analysed using quantitative PCR to understand the function during embryogenesis and its relationship with egg quality. Genes ccnb2 and pvalb1 showed significant differential expression between developmental stages and significant upregulation from blastula and somite stages, respectively. The comparison of spawning batches showed that the relative gene expression of genes ccnb2 , acta , tnnt3 and pvalb1 was significantly higher from the middle of the spawning season where phenotypic quality estimators establish the best egg quality. Moreover, a positive significant correlation was observed between quality estimators based on egg morphology and the genetic expression of genes acta and acta1 during somitogenesis. This study suggests that the combination of quality estimators, genetics and batch timing could help optimise reproductive protocols for commercial stocks of Atlantic cod. |
