| Autor: |
Caminos E; Department of Medical Science, Medical School of Albacete, Instituto de Biomedicina (IB-UCLM), University of Castilla-La Mancha, 02008 Albacete, Spain., López-López S; Department of Medical Science, Medical School of Albacete, Instituto de Biomedicina (IB-UCLM), University of Castilla-La Mancha, 02008 Albacete, Spain.; Consejo Superior de Investigaciones Científicas, and Research Unit, Complejo Hospitalario Universitario de Albacete, 02008 Albacete, Spain., Martinez-Galan JR; Department of Medical Science, Medical School of Albacete, Instituto de Biomedicina (IB-UCLM), University of Castilla-La Mancha, 02008 Albacete, Spain. |
| Abstrakt: |
Transient receptor potential canonical (TRPC) channels are calcium channels with diverse expression profiles and physiological implications in the retina. Neurons and glial cells of rat retinas with photoreceptor degeneration caused by retinitis pigmentosa (RP) exhibit basal calcium levels that are above those detected in healthy retinas. Inner retinal cells are the last to degenerate and are responsible for maintaining the activity of the visual cortex, even after complete loss of photoreceptors. We considered the possibility that TRPC1 and TRPC5 channels might be associated with both the high calcium levels and the delay in inner retinal degeneration. TRPC1 is known to mediate protective effects in neurodegenerative processes while TRPC5 promotes cell death. In order to comprehend the implications of these channels in RP, the co-localization and subsequent physical interaction between TRPC1 and TRPC5 in healthy retina (Sprague-Dawley rats) and degenerating (P23H-1, a model of RP) retina were detected by immunofluorescence and proximity ligation assays. There was an overlapping signal in the innermost retina of all animals where TRPC1 and TRPC5 physically interacted. This interaction increased significantly as photoreceptor loss progressed. Both channels function as TRPC1/5 heteromers in the healthy and damaged retina, with a marked function of TRPC1 in response to retinal degenerative mechanisms. Furthermore, our findings support that TRPC5 channels also function in partnership with STIM1 in Müller and retinal ganglion cells. These results suggest that an increase in TRPC1/5 heteromers may contribute to the slowing of the degeneration of the inner retina during the outer retinal degeneration. |
