| Autor: |
Kamal H; Department of Plant Pathology, Washington State University, Pullman, WA 99163, USA., Kotapati KV; Department of Plant Pathology, Washington State University, Pullman, WA 99163, USA., Tanaka K; Department of Plant Pathology, Washington State University, Pullman, WA 99163, USA., Pappu HR; Department of Plant Pathology, Washington State University, Pullman, WA 99163, USA. |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of molecular sciences [Int J Mol Sci] 2024 Jun 26; Vol. 25 (13). Date of Electronic Publication: 2024 Jun 26. |
| DOI: |
10.3390/ijms25136990 |
| Abstrakt: |
Potato mop-top virus (PMTV) is an emerging viral pathogen that causes tuber necrosis in potatoes. PMTV is composed of three single-stranded RNA segments: RNA1 encodes RNA-dependent RNA polymerase, RNA2 contains the coat protein (CP), and RNA3 harbors a triple gene block (TGB 1, TGB2, and TGB3). CP plays a role in viral transmission, while TGB is known to facilitate cell-to-cell and long-distance systemic movement. The role of CP in symptom development, specifically in the presence of TGB genes, was investigated using potato virus X (PVX) as a delivery vehicle to express PMTV genes in the model plant Nicotiana benthamiana . Plants expressing individual genes showed mild symptoms that included leaf curling and crumpling. Interestingly, symptom severity varied among plants infected with three different combinations: CP with TGB1, CP with TGB2, and CP with TGB3. Notably, the combination of CP and TGB3 induced a hypersensitive response, accompanied by stunted growth and downward curling and crumpling. These results suggest the potential role of TGB co-expressed with CP in symptom development during PMTV infection. Additionally, this study demonstrates the use of the PVX-based expression system as a valuable platform for assessing the role of unknown genes in viral pathogenicity. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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