Proteomic Characterization of a Lunasin-Enriched Soybean Extract Potentially Useful in the Treatment of Helicobacter pylori Infection.

Autor:	Franca-Oliveira G; Institute of Food Science Research (CIAL, CSIC-UAM, CEI UAM + CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain., Monreal Peinado S; Institute of Food Science Research (CIAL, CSIC-UAM, CEI UAM + CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain., Alves de Souza SM; Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.; Programa de Pós-Graduação em Nanobiossistemas, Universidade Federal do Rio de Janeiro, Duque de Caxias 25240-005, Brazil., Kalume DE; Laboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro 21041-250, Brazil., Ferraz de Souza TL; Faculdade de Farmácia, Universidade Federal do Rio de Janeiro, Rio de Janeiro 21941-902, Brazil.; Programa de Pós-Graduação em Nanobiossistemas, Universidade Federal do Rio de Janeiro, Duque de Caxias 25240-005, Brazil., Hernández-Ledesma B; Institute of Food Science Research (CIAL, CSIC-UAM, CEI UAM + CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain., Martinez-Rodriguez AJ; Institute of Food Science Research (CIAL, CSIC-UAM, CEI UAM + CSIC), Nicolás Cabrera 9, 28049 Madrid, Spain.
Jazyk:	angličtina
Zdroj:	Nutrients [Nutrients] 2024 Jun 27; Vol. 16 (13). Date of Electronic Publication: 2024 Jun 27.
DOI:	10.3390/nu16132056
Abstrakt:	Helicobacter pylori infection affects over 50% of the world's population and leads to chronic inflammation and gastric disorders, being the main pathogen correlated to gastric cancer development. Increasing antibiotic resistance levels are a major global concern and alternative treatments are needed. Soybean peptides and other compounds might be an alternative in the treatment to avoid, eradicate and/or control symptoms of H. pylori infection. This study aimed to characterize a lunasin-enriched soybean extract (LSE) using proteomics tools and to evaluate its antioxidant, anti-inflammatory and antibacterial properties against H. pylori infection. By LC-MS/MS analysis, 124 proteins were identified, with 2S albumin (lunasin and large-chain subunits) being the fourth most abundant protein (8.9%). Lunasin consists of 44 amino acid residues and an intramolecular disulfide bond. LSE at a low dose (0.0625 mg/mL) reduced ROS production in both H. pylori -infected and non-infected AGS gastric cells. This led to a significant reduction of 6.71% in the levels of pro-inflammatory interleukin (IL)-8. LSE also showed antibacterial activity against H. pylori , which can be attributed to other soybean proteins and phenolic compounds. Our findings suggest that LSE might be a promising alternative in the management of H. pylori infection and its associated symptoms. Competing Interests: The authors declare no conflicts of interest.
Databáze:	MEDLINE
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