Autor: |
Anjos LVSD; Agricultural Engineering and Soil, Faculty of Engineering, São Paulo State University (UNESP), Ilha Solteira 15385-000, SP, Brazil., Antonio GL; School of Agriculture, São Paulo State University (Unesp), Botucatu 18610-034, SP, Brazil., Fischer IH; São Paulo's Agency for Agribusiness Technology (APTA), Bauru Reginal, Bauru 17030-000, SP, Brazil., Goncalves ECP; São Paulo's Agency for Agribusiness Technology (APTA), Colina Center, Colina 14770-000, SP, Brazil., Scaloppi Junior EJ; Center of Rubber Tree and Agroforestry Systems, Agronomic Institute of Campinas (IAC), Votuporanga 15505-970, SP, Brazil., Furtado EL; School of Agriculture, São Paulo State University (Unesp), Botucatu 18610-034, SP, Brazil., de Oliveira TL; College of Agricultural and Technological Sciences, São Paulo State University (Unesp), Dracena 17900-000, SP, Brazil., Bello HN; College of Agricultural and Technological Sciences, São Paulo State University (Unesp), Dracena 17900-000, SP, Brazil., Firmino AC; College of Agricultural and Technological Sciences, São Paulo State University (Unesp), Dracena 17900-000, SP, Brazil. |
Abstrakt: |
The objective of this work was to evaluate the development of Davidiella sp. and its asexual form, Cladosporium sp., under different environmental conditions in the rubber tree ( Hevea brasiliensis ). Rubber tree leaves were inoculated with a spore suspension and kept in a humid chamber under different temperatures and wetness periods. The behavior of the fungi was evaluated using a scanning electron microscope (SEM) and an ultraviolet light microscope (UV). In the images obtained in SEM, four hours after inoculation of the fungus, it was possible to verify the germination and penetration of conidia at temperatures of 10 to 20 °C. The formation of conidiophores was verified from six hours after inoculation, indicating that it is in the reproductive period. In the sexual phase, in SEM, from four hours after inoculation, it was possible to verify the formation of small protuberances at temperatures between 10 and 20 °C. These black dots evolve into circular, protruding black spots, like the symptoms of black crust, with apparent spore formation on them. The data obtained from the UV analyses corroborate those from SEM, showing that the fungus has good development in its two phases between temperatures of 20 and 25 °C and that the period of wetness on the leaf can contribute to the initial development of the pathogen. |